Naslov
Kinetic spectrophotometric determination of N‐acetyl‐ L‐cysteine based on the reduction of copper(II)‐bathocuproine disulphonate complex

Autori
Biočić, Maja ; Kukoč Modun, Lea ; Stipišić, Martina ; Radić, Njegomir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka - 26 HRVATSKI SKUP KEMIČARA I KEMIJSKIH INŽENJERA Book of Abstracts - 26th Croatian Meeting of Chemists and Chemical Engineers / Galić, Nives ; Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2019, 115-115

ISBN
978-953-6894-67-3

Skup
26. hrvatski skup kemičara i kemijskih inženjera (26HSKIKI) ; 4. simpozij Vladimir Prelog

Mjesto i datum
Šibenik, Hrvatska, 09.04.2019. - 12.04.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
N‐acetyl‐L‐cysteine ; kinetic determination ; spectrophotometry

Sažetak
N‐acetyl‐L‐cysteine (acetylcysteine, NAC) is a synthetic aminothiol antioxidant used in medicine as a mucolytic agent with the aim of reducing the viscosity of pulmonary secretions in a variety of respiratory illnesses. Intravenous administration of NAC is used extensively in the management of paracetamol (acetaminophen) poisoning [1]. Kinetic spectrophotometry was employed for the development of a new method for determination of NAC in pharmaceutical formulations. The proposed method is based on a redox reaction in which the reduction of copper(II)‐bathocuproine disulphonate occurs by NAC with formation of orange coloured copper(I)‐bathocuproine disulphonate complex. This stable complex exhibits an absorption maximum at λ = 483 nm. The parameters of chemical reaction have been optimized. The proposed method was validated in terms of linearity range, limit of detection and limit of quantitation, selectivity, precision and accuracy. Using kinetic spectrophotometric measurements, initial rate and fixed time methods were utilized for constructing the calibration graphs. For the fixed time method, the graph was linear in the concentration range from 3 × 10–7 mol L –1 to 7 × 10 –5 mol L –1 with the limit of detection 8 × 10–8 mol L –1 obeying the equation y = 13140 x – 0.0005 with the correlation coefficient R2 = 0.9999. For the initial rate method, the graph was linear in the concentration range from 7 × 10–7 mol L –1 to 7 × 10 –5 mol L –1 with the limit of detection 2 × 10 –7 mol L –1 , equation y = 1.040 x + 3.220 and correlation coefficient R2 = 0.9991. No interferences were observed from the excipients commonly present in pharmaceutical formulations. The proposed methods were successfully applied for the determination of NAC in its commercial pharmaceutical formulations. The results were compared favourably with those obtained by their Pharmacopeial methods. References [1] G. S. Kelly, Altern. Med. Rev. 3 (1998) 114‐127.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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