Pregled bibliografske jedinice broj: 1190600
Oleanolic acid induces cytoprotective mitophagy in HCT116 human colon carcinoma cells
Oleanolic acid induces cytoprotective mitophagy in HCT116 human colon carcinoma cells // FEBS Open Bio 12(S1)
Lisabon, Portugal: Wiley-Blackwell, 2022. str. 206-206 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1190600 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Oleanolic acid induces cytoprotective mitophagy in
HCT116 human colon carcinoma cells
Autori
Šimić, Lidija ; Potočnjak, Iva ; Vukelić, Iva ; Batičić, Lara ; Domitrović, Robert
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FEBS Open Bio 12(S1)
/ - : Wiley-Blackwell, 2022, 206-206
Skup
The Biochemistry Global Summit
Mjesto i datum
Lisabon, Portugal, 06.07.2022. - 14.07.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
oleanolic acid ; HCT116 human colon cancer cells ; apoptosis ; autophagy, mitophagy, 5-Fluorouracil
Sažetak
Colorectal cancer is a disease that occupies a very high place in terms of its malignancy and mortality. Therefore, it is of high importance to discover compounds that can efficiently ameliorate the disease and reduce mortality. Oleanolic acid (OA), a natural triterpene compound, has been shown to possess beneficial health effects, including anticancer activity. However, the effect of OA on mitophagy in colon cancer cells is unknown. The aim of this study was to investigate the mechanism and the role of autophagy/mitophagy in the anticancer activity of OA in HCT116 human colon carcinoma cells, cell line commonly used in colon cancer studies, and therapeutic drug development. Additionally, we examined chemosensitization to 5fluorouracil (5FU). OA dosedependently reduced viability of HCT116 cells, with IC50 = 29.8 µM. The expression of cleaved caspase3 and poly (ADPribose) polymerase 1 (PARP) increased by OA treatment, suggesting that OA induces apoptosis in HCT116 cells. OA induced autophagy in cancer cells by increasing expression of Beclin1, Atg5 and microtubuleassociated protein 1A/1Blight chain 3 betaII (LC3BII), which was found to be protective. Induction of mitophagy was suggested by increased expression of p62 and PTEN induced kinase 1 (PINK1) and reduced expression of translocase of outer mitochondrial membrane 20 (TOMM20), which colocalized with LC3B. OA induced nuclear accumulation of forkhead box O3a (FOXO3a) and phoshoFOXO3a. The cytotoxic activity of OA coincided by suppression of the phosphoinositide 3kinase (PI3K)/Akt and extracellular regulated kinase 1/2 (ERK1/2) pathways and activation of AMPactivated protein kinase (AMPK), cJun Nterminal kinase 1 (JNK1), and p38. The results of the study show the cytotoxic activity of OA in HCT116 human colon carcinoma cells with concomitant induction of survival autophagy and mitophagy through modulation of key signaling pathways. Moreover, OA chemosensitized HCT116 cells to 5FU.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Farmacija
POVEZANOST RADA
Projekti:
MEDRI--uniri-biomed-18-30 - Interakcija lijekova i fitokemikalija in vitro i in vivo: uloga FOXO signalnog puta (Domitrović, Robert, MEDRI ) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-mladi-biomed-20-17 - Modulacija MEK-ERK MAPK signalnog puta u CP-induciranoj mitofagiji u bubrezima (Potočnjak, Iva, NadSve ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Lara Batičić
(autor)
Iva Potočnjak
(autor)
Lidija Šimić
(autor)
Iva Vukelić
(autor)
Robert Domitrović
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE