Naslov
Changes in anthropometric, biochemical, oxidative, and DNA damage parameters after 3-weeks-567-kcal- hospital-controlled-VLCD in severely obese patients with BMI ≥ 35 kg m−2

Autori
Ožvald, Ivan ; Božičević, Dragan ; Duh, Lidija ; Vinković Vrček, Ivana ; Domijan, Ana-Marija ; Milić, Mirta

Izvornik
Clinical nutrition ESPEN (2405-4577) 49 (2022); 319-327

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
alkaline comet assay ; DNA damage ; FPG oxidative comet assay ; VLCD ; Obesity

Sažetak
Background & aims: Severe obesity and its comorbidities relate to increased genomic instability/cancer risk. Obesity in Croatia is rapidly increasing, and long diets are sometimes the reason for obese to quit health improvement programs. A shorter diet with more strict calorie reduction could also lead to weight reduction and health improvements, but data are scarce. We tested for the first time if a very low-calorie diet (VLCD) can improve anthropometric, biochemical and genomic stability parameters in severely obese with BMI
35 kg m2. Methods: 22 participants were chosen among those regularly attending the hospital for obesity control, with no other previous treatment for bodyweight reduction. Under 24 h medical surveillance, patients received 3-weeks-567-kcal-hospital- controlled-VLCD composed of 50e60% complex carbohydrates, 20 e25% proteins, and 25e30% fat, with the attention to food carbo-glycemic index, in 3 meals freshly prepared in hospital. We analyzed changes in body weight, BMI, basal metabolism rate, waistehip ratio, visceral fat level, body fat mass, percent body fat, skeletal muscle mass, basal metabolism, energy intake, lipid profile, thyroid hormones, TSH, and genomic instability (alkaline and oxidative FPG comet assay) before and on the last VLCD day. Results: Diet caused BMI reduction (in average 3e4 BMI units' loss), excessive weight loss (between 10 and 35%), significant weight loss (average 9 kg, range 4.8e14.4 kg) and a significant decrease in glucose, insulin, urea, cholesterol, HDL-c, LDL-c, oxidative (FPG) and DNA damage (alkaline comet assay) levels. Conclusions: The diet can lead to
10% excessive weight loss, significant health, and genomic stability improvement, and keep severely obese interest in maintaining healthy habits. The study was registered at ClinicalTrials.gov as NCT05007171 (10.08.2021).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita, Farmacija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA