Pregled bibliografske jedinice broj: 1188908
Recognition of DNA:RNA hybrid and triplex structure by a series of benzothiazole ligands
Recognition of DNA:RNA hybrid and triplex structure by a series of benzothiazole ligands // 27th Croatian Meeting of Chemists and Chemical Engineers / Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna (ur.).
Zagreb: Hrvatsko kemijsko društvo, 2021. str. 44-44 (predavanje, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Recognition of DNA:RNA hybrid and triplex
structure by a series of benzothiazole ligands
Autori
Zonjić, Iva ; Tumir, Lidija Marija ; Šupljika, Filip ; Crnolatac, Ivo ; Racané, Livio ; Tomić, Sanja ; Radić Stojković Marijana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
27th Croatian Meeting of Chemists and Chemical Engineers
/ Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna - Zagreb : Hrvatsko kemijsko društvo, 2021, 44-44
Skup
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI)
Mjesto i datum
Veli Lošinj, Hrvatska, 05.10.2021. - 08.10.2021
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
DNA:RNA hybrids ; triplexes ; interactions ; benzothiazole ligands
Sažetak
DNA:RNA hybrids and triplexes are formed as intermediate structures during many biologically important processes such as DNA replication, transcription, telomere replication and modulation of structure and/or function of specific genes (antigene strategy for gene regulation).[1, 2, 3, 4] Interactions of an array of nucleic acid structures with a small series of benzothiazole ligands (1-9) were screened by competition dialysis assay (Figure 1). The main aim of this study was detection of benzothiazole structures with preferential binding to DNA:RNA hybrids and ATT triplex in regard to regular (non- hybrid) DNA and RNA duplexes and singlestranded forms. Complexes of nucleic acids and benzothiazoles, screened by this method, were then characterised by UV/Vis, fluorescence, circular dichroism (CD) spectroscopy, isothermal titration calorimetry and Compounds 1 and 6 showed the highest affinities toward 13 nucleic acid structures while 5, despite lower affinities, yielded higher selectivity among studied compounds. While both 6 and 1 exerted high binding affinities toward ATT, the latter showed stronger binding to rAdT hybrid whereas the former preferred dArU hybrid.
Izvorni jezik
Engleski
POVEZANOST RADA
Profili:
Filip Šupljika (autor)
Iva Zonjić (autor)
Livio Racane (autor)
Lidija-Marija Tumir (autor)
Ivo Crnolatac (autor)
Sanja Tomić (autor)