Pregled bibliografske jedinice broj: 1188891
Interactions of benzothiazole ligands with DNA:RNA hybrids and triplex structures
Interactions of benzothiazole ligands with DNA:RNA hybrids and triplex structures // IV. SIMPOZIJ SUPRAMOLEKULSKE KEMIJE Supramolecular Chemistry 2021
Zagreb, 2021. str. 1-1 (predavanje, domaća recenzija, sažetak, znanstveni)
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Naslov
Interactions of benzothiazole ligands with DNA:RNA
hybrids and triplex structures
Autori
Zonjić, Iva ; Tumir, Lidija Marija ; Šupljika, Filip ; Crnolatac, Ivo ; Racane, Livio ; Tomić, Sanja ; Radić Stojković, Marijana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
IV. SIMPOZIJ SUPRAMOLEKULSKE KEMIJE Supramolecular Chemistry 2021
/ - Zagreb, 2021, 1-1
Skup
4. simpozij supramolekulske kemije
Mjesto i datum
Zagreb, Hrvatska, 10.12.2021
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
DNA:RNA hybrids ; benzothiazole ligands ; ATT triplex ; interactions ; spectroscopy ; isothermal titration calorimetry
Sažetak
Nucleic acids are molecular targets for many drugs that are used in cancer therapy due to their essential functions in cells (replication, transcriptional and translational regulation, enzymatic reactions).1 Nucleic acid structures present a wide variety of shapes with varying major and minor groove widths that can be recognized by small molecules using a non-specific (mainly electrostatic) binding along the nucleic acid exterior, a specific groove binding and intercalation (insertion of planar aromatic molecules between base pairs).2 Interactions of an array of nucleic acid structures with a small series of benzothiazole ligands were screened by competition dialysis. The main aim of this study was the detection of benzothiazole-based moieties with selective binding or spectroscopic response to DNA:RNA hybrids and ATT triplex in regard to regular (non- hybrid) DNA and RNA duplexes and single-stranded forms. Complexes of nucleic acids and benzothiazoles, screened by this method, were then characterised by UV/Vis, fluorescence, circular dichroism (CD) spectroscopy and isothermal titration calorimetry. Compound 6 could differentiate between B- (binding of 6 dimers inside minor groove) and A-type (intercalation) helices by mode of binding while both 5 and 6 selectively stabilized ATT triplex regarding AT duplex. Compound 3 induced strong condensation- like changes in CD spectra of AT-rich DNA sequences.
Izvorni jezik
Engleski
POVEZANOST RADA
Profili:
Filip Šupljika (autor)
Iva Zonjić (autor)
Livio Racane (autor)
Lidija-Marija Tumir (autor)
Ivo Crnolatac (autor)
Sanja Tomić (autor)
Marijana Radić Stojković (autor)