Pregled bibliografske jedinice broj: 1188884
Influence of Oxygen on Adenosine A1 and A2a Receptor Gene Expression in Cerebral Blood Vessels of Sprague-Dawley Rats
Influence of Oxygen on Adenosine A1 and A2a Receptor Gene Expression in Cerebral Blood Vessels of Sprague-Dawley Rats // 4th International Student and 17th Bridges in Life Sciences Conferences / Prunchunas, Edward ; Vari, Sandor G. ; Laureova, Simona ; Vladar, Csaba (ur.).
Prag, 2022. str. 30-30 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1188884 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Influence of Oxygen on Adenosine A1 and A2a
Receptor Gene Expression in Cerebral Blood Vessels
of Sprague-Dawley Rats
Autori
Kovač, Lora ; Đambić, Vedran ; Šušnjara, Petar ; Kibel, Aleksandar, Drenjančević, Ines ; Mihaljević, Zrinka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
4th International Student and 17th Bridges in Life Sciences Conferences
/ Prunchunas, Edward ; Vari, Sandor G. ; Laureova, Simona ; Vladar, Csaba - Prag, 2022, 30-30
ISBN
978-615-6006-03-5
Skup
4th RECOOP International Student Conference (ISC) 17th RECOOP Bridges in Life Sciences Conferences
Mjesto i datum
Prag, Češka Republika, 07.04.2022. - 08.04.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
hyperbaric oxygenation, adenosine receptors, cerebral blood vessels, gene expression, Sprague-Dawley rats
Sažetak
Introduction: Hyperbaric oxygenation (HBO2) has the ability to alter protein expression, modulates signaling pathways, and influences vascular structure and function leading to changes in vasomotor responses. Adenosine receptors (AR) play an important role in the mechanism of action of oxygen as a vasoactive substance, the expression of which differs significantly between cell types. The aim of this study was to determine the expression of adenosine receptors' (A1R and A2aR) in cerebral blood vessels of Sprague-Dawley rats exposed to acute and intermittent HBO2. Methods: Male and female healthy Sprague-Dawley rats (N=18), 8-10 weeks old were divided into 3 groups: CTRL (control non-HBO2), A-HBO2 (exposed to single HBO2 at a pressure od 2 bars/ 2 hours) and 4D-HBO2 (animals exposed to HBO2 for 2 hours daily for 4 days, with sample isolation on 5th day). The rats were anesthetized with ketamine- chloride (3 ml/kg) and midazolam (0, 5 ml/kg) and decapitated. Afterwards, samples of surface brain blood vessels were used for qRT-PCR. Relative gene expression of A1R and A2aR genes was measured with HPRT1 as the housekeeping gene. Results: Acute exposure to HBO2 did not significantly alter A1R or A2aR gene expression. There was a significant increase in A1R i A2aR expression in the 4D-HBO2 group compared to CTRL. Discussion: High oxygen concentrations during HBO2 lead to changes in vascular reactivity mechanism. Adenosine A2aR are known to mediate a protective compensatory vasodilatory response to tissue hypoxia. Although A1R activation is known to cause vasoconstriction, some studies suggest that during hypoxia, A1R protects tissue from damage by reducing levels of ischemic mediator and by causing vasodilation. In addition, A1R can modulate A2aR expression. Also, A1R are known to be more sensitive to hypoxia. Conclusion: Intermittent exposure to hyperbaric oxygen acts as pseudohypoxia, resulting in a protective compensatory increase in adenosine A1R and A2aR gene expression.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
NadSve-Sveučilište J. J. Strossmayera u Osijeku-VIF 2021 - Uloga adenozinskih A1 i A2A receptora u mehanizmima vaskularne reaktivnosti nakon hiperbarične oksigenacije - molekularni pristup (Mihaljević, Zrinka, NadSve ) ( CroRIS)
NadSve-Sveučilište J. J. Strossmayera u Osijeku-IP 2020 - Uloga adenozinskih A1 i A2a receptora u promjenama vaskularne reaktivnosti nakon hiperbarične oksigenacije (Kibel, Aleksandar, NadSve ) ( CroRIS)
Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek
Profili:
Petar Šušnjara
(autor)
Zrinka Mihaljević
(autor)
Ines Drenjančević
(autor)
Vedran Đambić
(autor)
Aleksandar Kibel
(autor)