Naslov
Phenolic characterization and antioxidant capacity of buckthorn (Hippophae rhamnoides L.) leaf extracts obtained by microwave-assisted extraction

Autori
Pedisić, Sandra ; Lisica, Patricija ; Elez Garofulić, Ivona ; Pelaić, Zdenka ; Zorić, Zoran ; Dobrinčić, Ana ; Dragović-Uzelac, Verica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
35th International EFFoST Conference 2021: Healthy Individuals, Resilient Communities, and Global Food Security

Mjesto i datum
Lausanne, Švicarska, 01.11.2021. - 04.11.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sea buckthorn leaf ; microwave-assisted extraction ; phenolics ; antioxidant capacity

Sažetak
Sea buckthorn (Hippophae rhamnoides L.) is rich source of bioactives such as phenolics and lipophilic compounds and used as food and medicinal plant. Higher phenolic contents (PC) and antioxidant capacities (AC) were determined in sea buckthorn leaves (SBL) than in berries. Phenolic extraction from plants is the first step in utilization of these compounds and more efficient extraction processes such as microwave assisted extraction (MAE) have been developed in recent years due to reduction of extraction time and solvent. Therefore, the aim of this study was to evaluate the PC and AC in SBL extracts obtained under different MAE conditions of microwave power (300, 500 and 700 W) and extraction time (5, 10 and 15 minutes) at 60 ºC using 70% ethanol (v/v) as the extraction solvent. Phenolic profile of SBL extracts was determined using UPLC-MS2 and AC by ORAC method, respectively. A total of 30 phenolic compounds were determined comprising flavonols (FOL), flavones (FON), flavan-3-ols (F-3-OL) and phenolic acids (PA). The FOL (372.35 to 538.69 mg 10-2g-1 dm) showed to be the predominant phenolic group in SBL represented by rutin and kaempferol as the most abundant. Considerably lower content of total PA (71.29-132.29 mg 10-2g-1 dm), FON (7.99-22.49 mg 10-2g-1 dm) and F-3-ON (2.70-3.72 mg 10-2g-1 dm) were determined. The all SBL extracts had high AC (8779.17-9186.42 μmol TE 10- 2g-1 dm). Applied MAE conditions significantly affected the concentration of FOL, FON, PA and AC. Generally, the highest content of FOL, FON and AC was observed in extracts obtained under microwave power of 500 W and extraction time of 15 minutes, while the highest content of PA was observed under microwave power of 700 W and shorter extraction time of 5 minutes. Therefore, MAE is effective method for the production of valuable extracts from SBL with potential beneficial applications.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA