Naslov
A multifunctional strontium/silver-co-substituted hydroxyapatite derived from biogenic source as antibacterial biomaterial

Autori
Ressler, Antonia ; Ivanković, Tomislav ; Polak, Bruno ; Ivanišević, Irena ; Kovačić, Marin ; Urlić, Inga ; Hussainova, Irina ; Ivanković, Hrvoje

Izvornik
Ceramics international (0272-8842) 48 (2022), 13; 18361-18373

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
co-substitution ; Escherichia coli ; hydroxyapatite ; Staphylococcus aureus ; strontium ; silver

Sažetak
The bioactive properties of hydroxyapatite (HAp) facilitate bone regeneration, however, its physico-chemical and bioactive properties can be further enhanced by ionic substitutions within the crystalline lattice. In this work, substitution with Sr2+ and Ag+ ions was investigated for the improvement of osseointegration and antibacterial activity in potential treatment of traumatic bone injuries. A series of single substituted HAp with Sr2+ or Ag+ ions and Ag/Sr-co-substituted HAp with different degrees of substitution (0, 1, 2.5, and 5 mol%) were obtained by wet precipitation from cuttlefish bone. Rietveld refinement indicated successful Ca2+ substitution by increasing cell parameters due to the larger ionic radii of Sr2+ and Ag+ compared to Ca2+, which was confirmed by elemental mapping showing uniform distribution of substituent cations. Characterization of the zeta-potential of Ag/Sr-co-substituted HAp showed negatively charged populations, at potentials not lower than −15 mV. Increasing the degree of substitution resulted in decreasing zeta-potential. The higher absorption capacity of bovine serum albumin was determined on Ag/Sr-co-substituted HAp powders as compared to non-substituted HAp. All Ag-substituted HAps have shown an antibacterial activity towards Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, as determined by inhibition zone, viability analysis and scanning electron microscopy. In addition, non-cytotoxicity towards human cells was confirmed by in vitro tests with embryonic kidney 293 and mesenchymal stem cells.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

POVEZANOST RADA