Extended prophylactic effect of N-tert-Butyl-α-phenylnitron against oxidative/nitrosative damage caused by the DNA-hypomethylating drug 5-Azacytidine in the rat placenta

Sobočan, Nikola; Himelreich-Perić, Marta; Katušić- Bojanac, Ana; Krasić, Jure; Sinčić, Nino; Majić, Željka; Jurić-Lekić, Gordana; Šerman, Ljiljana; Marić, Andreja; Ježek, Davor; Bulić-Jakuš, Floriana

doi:10.3390/ijms23020603

Pregled bibliografske jedinice broj: 1183824

Extended prophylactic effect of N-tert-Butyl-α- phenylnitron against oxidative/nitrosative damage caused by the DNA-hypomethylating drug 5- Azacytidine in the rat placenta

Sobočan, Nikola; Himelreich-Perić, Marta; Katušić- Bojanac, Ana; Krasić, Jure; Sinčić, Nino; Majić, Željka; Jurić-Lekić, Gordana; Šerman, Ljiljana; Marić, Andreja; Ježek, Davor; Bulić-Jakuš, Floriana

Extended prophylactic effect of N-tert-Butyl-α- phenylnitron against oxidative/nitrosative damage caused by the DNA-hypomethylating drug 5- Azacytidine in the rat placenta // International journal of molecular sciences, 23 (2022), 2; 603, 14 doi:10.3390/ijms23020603 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1183824 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Extended prophylactic effect of N-tert-Butyl-α- phenylnitron against oxidative/nitrosative damage caused by the DNA-hypomethylating drug 5- Azacytidine in the rat placenta

Autori
Sobočan, Nikola ; Himelreich-Perić, Marta ; Katušić- Bojanac, Ana ; Krasić, Jure ; Sinčić, Nino ; Majić, Željka ; Jurić-Lekić, Gordana ; Šerman, Ljiljana ; Marić, Andreja ; Ježek, Davor ; Bulić-Jakuš, Floriana

Izvornik
International journal of molecular sciences (1422-0067) 23 (2022), 2; 603, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
5-azacytidine ; N-tert-Butyl-α-phenylnitron ; PBN ; placenta ; oxoguanosine ; nitrotyrosine ; oxidative stress

Sažetak
Antioxidant N-tert-Butyl-α-phenylnitron (PBN) partly protected embryos from the negative effects of a DNA demethylating drug 5-azacytidine during pregnancy. Our aim was to investigate PBN’s impact on the placenta. Fischer rat dams were treated on gestation days (GD) 12 and 13 by PBN (40 mg/kg), followed by 5azaC (5 mg/kg) after one hour. Global methylation was assessed by pyrosequencing. Numerical density was calculated from immunohistochemical expression in single cells for proliferating (PCNA), oxidative (oxoguanosine) and nitrosative (nitrotyrosine) activity. Results were compared with the PBN-treated and control rats. PBN-pretreatment significantly increased placental weight at GD15 and GD20, diminished by 5azaC, and diminished apoptosis in GD 20 placentas caused by 5azaC. Oxoguanosine expression in placentas of 5azaC-treated dams was especially high in the placental labyrinth on GD 15, while PBN- pretreatment lowered its expression on GD 15 and GD 20 in both the labyrinth and basal layer. 5azaC enhanced nitrotyrosine level in the labyrinth of both gestational stages, while PBN- pretreatment lowered it. We conclude that PBN exerted its prophylactic activity against DNA hypomethylating agent 5azaC in the placenta through free radical scavenging, especially in the labyrinthine part of the placenta until the last day of pregnancy.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA

Projekti:
--KK.01.1.1.01.0008 - Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platormi i potencijala (CERRM) (Ježek, Davor; Vukičević, Slobodan) ( CroRIS)

Ustanove:
Klinička bolnica "Merkur",
Medicinski fakultet, Zagreb

Profili:

Gordana Jurić-Lekić (autor)