Pregled bibliografske jedinice broj: 1183082
Recognition of ATT Triplex and DNA:RNA Hybrid Structures by Benzothiazole Ligands
Recognition of ATT Triplex and DNA:RNA Hybrid Structures by Benzothiazole Ligands // Biomolecules, 12 (2022), 3; 1600173, 20 doi:10.3390/biom12030374 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1183082 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Recognition of ATT Triplex and DNA:RNA Hybrid
Structures by Benzothiazole Ligands
Autori
Zonjić, Iva ; Tumir, Lidija-Marija ; Crnolatac, Ivo ; Šupljika, Filip ; Racané, Livio ; Tomić, Sanja ; Radić Stojković, Marijana
Izvornik
Biomolecules (2218-273X) 12
(2022), 3;
1600173, 20
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
benzothiazoles ; competition dialysis ; DNA:RNA hybrids ; ATT triplex ; circular dichroism spectroscopy ; RNase H
Sažetak
Interactions of an array of nucleic acid structures with a small series of benzothiazole ligands (bis-benzothiazolyl-pyridines—group 1, 2- thienyl/2-benzothienyl-substituted 6-(2- imidazolinyl) benzothiazoles—group 2, and three 2- aryl/heteroaryl-substituted 6-(2- imidazolinyl)benzothiazoles—group 3) were screened by competition dialysis. Due to the involvement of DNA:RNA hybrids and triplex helices in many essential functions in cells, this study’s main aim is to detect benzothiazole based moieties with selective binding or spectroscopic response to these nucleic structures compared to regular (non- hybrid) DNA and RNA duplexes and single-stranded forms. Complexes of nucleic acids and benzothiazoles, selected by this method, were characterized by UV/Vis, fluorescence and circular dichroism (CD) spectroscopy, isothermal titration calorimetry, and molecular modeling. Two compounds (1 and 6) from groups 1 and 2 demonstrated the highest affinities against 13 nucleic acid structures, while another compound (5) from group 2, despite lower affinities, yielded higher selectivity among studied compounds. Compound 1 significantly inhibited RNase H. Compound 6 could differentiate between B- (binding of 6 dimers inside minor groove) and A- type (intercalation) helices by an induced CD signal, while both 5 and 6 selectively stabilized ATT triplex in regard to AT duplex. Compound 3 induced strong condensation-like changes in CD spectra of AT-rich DNA sequences.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
--IP-2018-01-4694 - Molekularno prepoznavanje DNA:RNA hibridnih i višelančanih struktura u bioanalitičkim i in vitro sustavima (DNARNAHyB-MolBio) (Radić Stojković, Marijana) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Tekstilno-tehnološki fakultet, Zagreb
Profili:
Filip Šupljika (autor)
Iva Zonjić (autor)
Livio Racane (autor)
Lidija-Marija Tumir (autor)
Ivo Crnolatac (autor)
Sanja Tomić (autor)
Marijana Radić Stojković (autor)
Poveznice na cjeloviti tekst rada:
Pristup cjelovitom tekstu rada doi www.mdpi.com doi.org fulir.irb.hrCitiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE