Naslov
New spirit of an old technique: Characterization of lipid phase transitions via UV/Vis spectroscopy

Autori
Maleš, Petra ; Brkljača, Zlatko ; Domazet Jurašin, Darija ; Bakarić, Danijela

Izvornik
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (1386-1425) 272 (2022); 121013, 7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
UV/Vis spectroscopy ; 1, 2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) ; Cholesterol ; Lipid phase transitions ; Differential spectral analysis (DSA) ; Multivariate curve analysis (MCA)

Sažetak
One of the advantages of investigating lipid phase transitions by thermoanalytical techniques such as DSC is manifested in the proportionality of the signal strength on a DSC curve, attributed to a particular thermotropic event, and its cooperativity degree. Accordingly, the pretransition of 1, 2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC) is less noticeable than its main phase transition ; as a matter of fact, when DSC measurements are performed at low heating rate, such low-cooperativity phase transition could go (almost) unnoticed. The aim of this work is to present temperature-dependent UV/Vis spectroscopy, based on a temperature-dependent change in DPPC suspension turbidity, as a technique applicable for determination of lipid phase transition temperatures. Multivariate analyzes of the acquired UV/Vis spectra show that phase transitions of the low-cooperativity degree, such as pretransitions, can be identified with the same certainty as transitions of a high- cooperativity degree.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA