Naslov
Plasma levels and tissue expression of soluble TGFβrIII receptor in women with early-stage breast cancer and in healthy women: a prospective observational study

Autori
Grgurević, Lovorka ; Novak, Ruđer ; Trkulja, Vladimir ; Hrkač, Stela ; Salai, Grgur ; Bilandžić, Joško ; Ferhatović Hamzić, Lejla ; Milas, Ivan ; Vučemilo, Tiha ; Perić Balja, Melita ; Bilić, Karmen

Izvornik
Journal of translational medicine (1479-5876) 18 (2020); 478, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Soluble TGFßrIII ; Betaglycan ; Breast cancer ; Shedding

Sažetak
Ackground: Mammary carcinogenesis is partly regulated by the transforming growth factor beta (TGFβ) signaling pathway. Its function in cancer progression and metastasis is highly dependent on disease stage, and it is likely modulated by the ratio of membrane-bound vs. soluble TGFβrIII (sTGFβrIII). In this prospective observational study, we assessed tissue expression and plasma levels of sTGFβrIII in healthy women, women with benign breast lesions and in early-stage breast cancer patients. Methods: In a preliminary study, plasma sTGFβrIII levels were determined in 13 healthy women (age 19–40 years) at diferent phases of the ovarian cycle, and in 15 patients (age 35–75 years) at diferent times of the day. The main study assessed plasma concentrations of sTGFβrIII in: (i) 158 healthy women in whom breast lesions were excluded ; (ii) 65 women with benign breast lesions ; (iii) 147 women with newly diagnosed breast cancer classifed as American Joint Committee on Cancer (AJCC) stages 0 to IIB. Completers provided blood samples before surgery and at 10–30 and 160–180 days after surgery. Plasma sTGFβrIII concentrations were determined using an indirect ELISA kit. Part of the removed tissues underwent immunohistochemical (IHC) staining and analysis of tissue TGFβrIII expression. Results: There appeared no relevant variations in plasma sTGFßrIII levels at diferent times of the day or diferent ovarian cycle phases. Before surgery, breast cancer patients had somewhat higher sTGFβrIII than healthy women, or those with benign breast lesions (by 14.5 and 26 ng/mL, respectively), with a tendency of larger diferences at higher age. This correlated with lower expression of TGFβrIII in breast cancer vs. healthy tissue samples. At 160–180 days after surgery, plasma sTGFβrIII levels in breast cancer patients declined by 23–26 ng/mL. Conclusions: Plasma sTGFβrIII levels do not seem to relevantly vary during the day or the ovarian cycle. The coinciding higher plasma levels in newly diagnosed cancer patients than in healthy subjects and lower TGFβrIII expression in the malignant than in healthy breast tissue suggest ectodomain shedding as a source of circulating sTGFβrIII. Decline in plasma levels after tumor removal supports such a view.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA