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Pregled bibliografske jedinice broj: 1178786

Are volatile isolates containing glucosinolate breakdown products overlooked in testing the activity against mycotoxigenic Penicillium verrucosum?


Ramić, Dina; Vrca, Ivana; Bilušić, Tea; Blažević, Ivica; Smole Možina, Sonja
Are volatile isolates containing glucosinolate breakdown products overlooked in testing the activity against mycotoxigenic Penicillium verrucosum? // Acta Microbiologica et Immunologica Hungarica vol.68, suppl.1 : Abstracts of the 6th Central European Forum for Microbiology / Marialigeti, K. ; Dobay, O. (ur.).
Kecskemét, Mađarska: Hungarian Society for Microbiology, 2021. str. 107-108 (poster, međunarodna recenzija, prošireni sažetak, znanstveni)


CROSBI ID: 1178786 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Are volatile isolates containing glucosinolate breakdown products overlooked in testing the activity against mycotoxigenic Penicillium verrucosum?

Autori
Ramić, Dina ; Vrca, Ivana ; Bilušić, Tea ; Blažević, Ivica ; Smole Možina, Sonja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, prošireni sažetak, znanstveni

Izvornik
Acta Microbiologica et Immunologica Hungarica vol.68, suppl.1 : Abstracts of the 6th Central European Forum for Microbiology / Marialigeti, K. ; Dobay, O. - : Hungarian Society for Microbiology, 2021, 107-108

Skup
6th Central European Forum for Microbiology

Mjesto i datum
Kecskemét, Mađarska, 13.10.2021. - 15.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
penicillium verrucosum ; antifungal activity ; nasturtium volatile isolate ; benzyl isothiocyanate ; 2-phenylacetonirile

Sažetak
Moulds that cause food spoilage are a huge problem in the food industry, especially mycotoxin- producing moulds, which pose a major health risk to food consumers. Penicillium verrucosum is one of the major producers of ochratoxin A (OTA) in food. International Agency for Research on Cancer (IARC) classifies OTA as a nephrotoxic, neurotoxic, hepatoxic, teratogenic, genotoxic, immunotoxic, embryotoxic, and carcinogenic toxin. Traditionally, mould growth and OTA production in foods have been controlled by the use of various chemical additives, but nowadays there is an increasing demand from consumers to avoid chemical food additives, which has prompted researchers to search for new safe and biodegradable preservatives. Many studies indicate that essential oils (EOs) and volatile isolates (VIs) rich in low molecular weight phenolic and terpenoid compounds are promising alternative antifungal agents, but there haven’t been many studies showing the antifungal activity of EOs and VIs rich in glucosinolate breakdown products, such as isothiocyanates, nitriles, epithionitriles, and thiocyanates. For that purpose, the antifungal activity of nasturtium (Tropaeolum majus L.) VI was tested. The nasturtium seeds powder was subjected to microwave hydrodiffusion and gravity (MHG) for 15 min at a power of 500 W using an ETHOS X device, and further hydrodistillated with Clevenger system for 2.5 hours to obtain a VI. The chemical composition of nasturtium VI was determined by GC-MS/MS, while the minimal inhibitory concentrations (MIC) of nasturtium VI and its pure compounds (benzyl isothiocyanate, 2- phenylacetonitrile) against P. verrucosum were determined by microdilution method. GC-MS/MS analysis revealed that nasturtium VI was mainly composed of benzyl isothiocyanate (54.35%), and 2- phenylacetonitrile (37%). Among these compounds, nasturtium VI was the most effective with MIC value of 0.05 mg/mL. Excellent antifungal activity was also found for pure benzyl isothiocyanate with MIC value of 0.10 mg/mL, while pure 2- phenylacetonitrile was 8 fold lower with MIC value of 0.8 mg/mL. For comparison, Jeršek et al. (2014) found that oregano EO, mainly composed of phenolic compounds thymol (4.5%) and carvacrol (53.4%), had a MIC of 1.09 mg/mL. The most potent of these compounds was thymol with a MIC of 0.12 mg/mL, while carvacrol had a MIC of 0.38 mg/mL. Thus, it can be concluded that nasturtium VI, which is rich in glucosinolate breakdown products (benzyl isothiocyanate, 2-phenylacetonitrile), has ca. 20 times better antifungal activity against P. verrucosum than oregano EO. It can be assumed that isothiocyanates are the main compounds that confer excellent antifungal activity to nasturtium VI. This result indicates that VIs rich in glucosinolate breakdown products, which are not typically used in mould control studies, are a promising alternative to phenol-rich EOs. Isothiocyanates are lipophilic and therefore can react with membrane-bound enzymes, inhibit fungal growth and cause cell death. In addition, benzyl isothiocyanate is known for its inhibitory effect on mycotoxin production, such as AME, AOH, ALT and TEN (Wang et al., 2020). Therefore, it is necessary to determine the effect of nasturtium VI, benzyl isothiocyanate and 2-phenylacetonitrile on mycotoxin production of P. verrucosum. It is also important to test synergism between the glucosinolate breakdown products in the further studies.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija, Prehrambena tehnologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-1316 - Biljke kao izvor bioaktivnih sumporovih spojeva te njihova sposobnost hiperakumulacije metala (BioSMe) (Blažević, Ivica, HRZZ - 2016-06) ( CroRIS)
HRZZ-DOK-2018-01-9627 - Biljke kao izvor bioaktivnih sumporovih spojeva te njihova sposobnost hiperakumulacije metala (BioSMe) (Bilušić, Tea, HRZZ - 2018-02) ( CroRIS)

Ustanove:
Kemijsko-tehnološki fakultet, Split

Profili:

Avatar Url Ivica Blažević (autor)

Avatar Url Ivana Vrca (autor)

Avatar Url Tea Bilušić (autor)

Citiraj ovu publikaciju:

Ramić, Dina; Vrca, Ivana; Bilušić, Tea; Blažević, Ivica; Smole Možina, Sonja
Are volatile isolates containing glucosinolate breakdown products overlooked in testing the activity against mycotoxigenic Penicillium verrucosum? // Acta Microbiologica et Immunologica Hungarica vol.68, suppl.1 : Abstracts of the 6th Central European Forum for Microbiology / Marialigeti, K. ; Dobay, O. (ur.).
Kecskemét, Mađarska: Hungarian Society for Microbiology, 2021. str. 107-108 (poster, međunarodna recenzija, prošireni sažetak, znanstveni)
Ramić, D., Vrca, I., Bilušić, T., Blažević, I. & Smole Možina, S. (2021) Are volatile isolates containing glucosinolate breakdown products overlooked in testing the activity against mycotoxigenic Penicillium verrucosum?. U: Marialigeti, K. & Dobay, O. (ur.)Acta Microbiologica et Immunologica Hungarica vol.68, suppl.1 : Abstracts of the 6th Central European Forum for Microbiology.
@article{article, author = {Rami\'{c}, Dina and Vrca, Ivana and Bilu\v{s}i\'{c}, Tea and Bla\v{z}evi\'{c}, Ivica and Smole Mo\v{z}ina, Sonja}, year = {2021}, pages = {107-108}, keywords = {penicillium verrucosum, antifungal activity, nasturtium volatile isolate, benzyl isothiocyanate, 2-phenylacetonirile}, title = {Are volatile isolates containing glucosinolate breakdown products overlooked in testing the activity against mycotoxigenic Penicillium verrucosum?}, keyword = {penicillium verrucosum, antifungal activity, nasturtium volatile isolate, benzyl isothiocyanate, 2-phenylacetonirile}, publisher = {Hungarian Society for Microbiology}, publisherplace = {Kecskem\'{e}t, Ma\djarska} }
@article{article, author = {Rami\'{c}, Dina and Vrca, Ivana and Bilu\v{s}i\'{c}, Tea and Bla\v{z}evi\'{c}, Ivica and Smole Mo\v{z}ina, Sonja}, year = {2021}, pages = {107-108}, keywords = {penicillium verrucosum, antifungal activity, nasturtium volatile isolate, benzyl isothiocyanate, 2-phenylacetonirile}, title = {Are volatile isolates containing glucosinolate breakdown products overlooked in testing the activity against mycotoxigenic Penicillium verrucosum?}, keyword = {penicillium verrucosum, antifungal activity, nasturtium volatile isolate, benzyl isothiocyanate, 2-phenylacetonirile}, publisher = {Hungarian Society for Microbiology}, publisherplace = {Kecskem\'{e}t, Ma\djarska} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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