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Pregled bibliografske jedinice broj: 1176586

Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses

Schaer, Jessica; Cvetnic, Zeljko; Sukalic, Tomislav; Dörig, Sven; Grisiger, Martin; Iscaro, Carmen; Feliziani, Francesco; Pfeifer, Folke; Origgi, Francesco; Zanoni, Reto Giacomo; Abril, Carlos Eduardo
Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses // Pathogens, 11 (2022), 2; 129, 21 doi:10.3390/pathogens11020129 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1176586 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses

Autori
Schaer, Jessica ; Cvetnic, Zeljko ; Sukalic, Tomislav ; Dörig, Sven ; Grisiger, Martin ; Iscaro, Carmen ; Feliziani, Francesco ; Pfeifer, Folke ; Origgi, Francesco ; Zanoni, Reto Giacomo ; Abril, Carlos Eduardo

Izvornik
Pathogens (2076-0817) 11 (2022), 2; 129, 21

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
small ruminant lentivirus ; Maedi-Visna ; caprine arthritis-encephalitis ; diagnosis ; serological methods ; molecular methods ; ELISA ; nested real-time PCR

Sažetak
Small ruminant lentiviruses (SRLVs), i.e., CAEV and MVV, cause insidious infections with life-long persistence and a slowly progressive disease, impairing both animal welfare and productivity in affected herds. The complex diagnosis of SRLVs currently combines serological methods including whole-virus and peptide-based ELISAs and Immunoblot. To improve the current diagnostic protocol, we analyzed 290 sera of animals originating from different European countries in parallel with three commercial screening ELISAs, Immunoblot as a confirmatory assay and five SU5 peptide ELISAs for genotype differentiation. A newly developed nested real-time PCR was carried out for the detection and genotype differentiation of the virus. Using a heat-map display of the combined results, the drawbacks of the current techniques were graphically visualized and quantified. The immunoblot and the SU5-ELISAs exhibited either unsatisfactory sensitivity or insufficient reliability in the differentiation of the causative viral genotype, respectively. The new truth standard was the concordance of the results of two out of three screening ELISAs and the PCR results for serologically false negative samples along with genotype differentiation. Wholevirus antigen-based ELISA showed the highest sensitivity (92.2%) and specificity (98.9%) among the screening tests, whereas PCR exhibited a sensitivity of 75%.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA

Ustanove:
Hrvatski veterinarski institut, Zagreb

Profili:

Avatar Url Tomislav Sukalić (autor)

Avatar Url Željko Cvetnić (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.mdpi.com

Citiraj ovu publikaciju:

Schaer, Jessica; Cvetnic, Zeljko; Sukalic, Tomislav; Dörig, Sven; Grisiger, Martin; Iscaro, Carmen; Feliziani, Francesco; Pfeifer, Folke; Origgi, Francesco; Zanoni, Reto Giacomo; Abril, Carlos Eduardo
Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses // Pathogens, 11 (2022), 2; 129, 21 doi:10.3390/pathogens11020129 (međunarodna recenzija, članak, znanstveni)
Schaer, J., Cvetnic, Z., Sukalic, T., Dörig, S., Grisiger, M., Iscaro, C., Feliziani, F., Pfeifer, F., Origgi, F., Zanoni, R. & Abril, C. (2022) Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses. Pathogens, 11 (2), 129, 21 doi:10.3390/pathogens11020129.
@article{article, author = {Schaer, Jessica and Cvetnic, Zeljko and Sukalic, Tomislav and D\"{o}rig, Sven and Grisiger, Martin and Iscaro, Carmen and Feliziani, Francesco and Pfeifer, Folke and Origgi, Francesco and Zanoni, Reto Giacomo and Abril, Carlos Eduardo}, year = {2022}, pages = {21}, DOI = {10.3390/pathogens11020129}, chapter = {129}, keywords = {small ruminant lentivirus, Maedi-Visna, caprine arthritis-encephalitis, diagnosis, serological methods, molecular methods, ELISA, nested real-time PCR}, journal = {Pathogens}, doi = {10.3390/pathogens11020129}, volume = {11}, number = {2}, issn = {2076-0817}, title = {Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses}, keyword = {small ruminant lentivirus, Maedi-Visna, caprine arthritis-encephalitis, diagnosis, serological methods, molecular methods, ELISA, nested real-time PCR}, chapternumber = {129} }
@article{article, author = {Schaer, Jessica and Cvetnic, Zeljko and Sukalic, Tomislav and D\"{o}rig, Sven and Grisiger, Martin and Iscaro, Carmen and Feliziani, Francesco and Pfeifer, Folke and Origgi, Francesco and Zanoni, Reto Giacomo and Abril, Carlos Eduardo}, year = {2022}, pages = {21}, DOI = {10.3390/pathogens11020129}, chapter = {129}, keywords = {small ruminant lentivirus, Maedi-Visna, caprine arthritis-encephalitis, diagnosis, serological methods, molecular methods, ELISA, nested real-time PCR}, journal = {Pathogens}, doi = {10.3390/pathogens11020129}, volume = {11}, number = {2}, issn = {2076-0817}, title = {Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses}, keyword = {small ruminant lentivirus, Maedi-Visna, caprine arthritis-encephalitis, diagnosis, serological methods, molecular methods, ELISA, nested real-time PCR}, chapternumber = {129} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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