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Pregled bibliografske jedinice broj: 1173867

Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency


Nestić, Davor; Hozić, Amela; Brkljača, Zlatko; Butorac, Ana; Pažur, Kristijan; Jullienne, Betsy; Cindrić, Mario; Ambriović-Ristov, Andreja; Benihoud, Karim; Majhen, Dragomira
Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency // Life sciences, 291 (2022), 120116, 9 doi:10.1016/j.lfs.2021.120116 (međunarodna recenzija, članak, znanstveni)


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Naslov
Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency

Autori
Nestić, Davor ; Hozić, Amela ; Brkljača, Zlatko ; Butorac, Ana ; Pažur, Kristijan ; Jullienne, Betsy ; Cindrić, Mario ; Ambriović-Ristov, Andreja ; Benihoud, Karim ; Majhen, Dragomira

Izvornik
Life sciences (0024-3205) 291 (2022); 120116, 9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Adenovirus vector ; NGR retargeting peptide ; CD13 ; Integrin

Sažetak
Aims Adenoviruses that have CNGRCVSGCAGRC peptide inserted into fiber (AdFNGR) or hexon (AdHNGR) protein, respectively, showed increased transduction of endothelial cells. In this study we investigated if cysteines within the CNGRCVSGCAGRC sequence inserted into Ad5 fiber or hexon protein form disulfide bond(s) and whether they play a role in retargeting potential of AdFNGR and AdHNGR. Methods Transduction efficiency of adenoviruses was done by counting infected cells under the microscope. Adenovirus attachment and internalization were measured by qPCR. Flow cytometry was used to evaluate the expression of CD13 and integrins. Gene knockdown was achieved by transfection of small interfering RNA. Mass spectrometry was used for determining disulfide bonds in adenovirus fiber and hexon protein. Molecular modeling was use to predict interaction of CNGRCVSGCAGRC peptide and CD13. Key findings AdFNGR and AdHNGR attach better to CD13 and/or αvβ3 integrin-positive cells than Adwt. Reducing disulfide bonds using DTT decreased transduction efficiency and attachment of both AdFNGR and AdHNGR. Cysteins from CNGRCVSGCAGRC peptide within AdHNGR do not form disulfide bonds. Knockdown of αvβ3 integrin reduced increased transduction efficiency of both AdFNGR and AdHNGR, while CD13 knockdown had no effect, indicating that retargeting properties of these viruses rely mainly on αvβ3 integrin expression. Significance Insertion site of NGR-containing peptides as well as NGR flanking residues are critical for receptor binding affinity/specificity and transduction efficiency of NGR retargeted adenoviral vector

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2019-04-6048 - Endocitoza adenovirusa i urođeni imunosni odgovor (AdenoIN) (Majhen, Dragomira, HRZZ - 2019-04) ( CroRIS)
HRZZ-IP-2019-04-1577 - Integrin alpha V beta 5-povezane fokalne i retikularne adhezije u melanomu (AdMeFoRe) (Ambriović Ristov, Andreja, HRZZ - 2019-04) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com doi.org

Citiraj ovu publikaciju:

Nestić, Davor; Hozić, Amela; Brkljača, Zlatko; Butorac, Ana; Pažur, Kristijan; Jullienne, Betsy; Cindrić, Mario; Ambriović-Ristov, Andreja; Benihoud, Karim; Majhen, Dragomira
Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency // Life sciences, 291 (2022), 120116, 9 doi:10.1016/j.lfs.2021.120116 (međunarodna recenzija, članak, znanstveni)
Nestić, D., Hozić, A., Brkljača, Z., Butorac, A., Pažur, K., Jullienne, B., Cindrić, M., Ambriović-Ristov, A., Benihoud, K. & Majhen, D. (2022) Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency. Life sciences, 291, 120116, 9 doi:10.1016/j.lfs.2021.120116.
@article{article, author = {Nesti\'{c}, Davor and Hozi\'{c}, Amela and Brklja\v{c}a, Zlatko and Butorac, Ana and Pa\v{z}ur, Kristijan and Jullienne, Betsy and Cindri\'{c}, Mario and Ambriovi\'{c}-Ristov, Andreja and Benihoud, Karim and Majhen, Dragomira}, year = {2022}, pages = {9}, DOI = {10.1016/j.lfs.2021.120116}, chapter = {120116}, keywords = {Adenovirus vector, NGR retargeting peptide, CD13, Integrin}, journal = {Life sciences}, doi = {10.1016/j.lfs.2021.120116}, volume = {291}, issn = {0024-3205}, title = {Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency}, keyword = {Adenovirus vector, NGR retargeting peptide, CD13, Integrin}, chapternumber = {120116} }
@article{article, author = {Nesti\'{c}, Davor and Hozi\'{c}, Amela and Brklja\v{c}a, Zlatko and Butorac, Ana and Pa\v{z}ur, Kristijan and Jullienne, Betsy and Cindri\'{c}, Mario and Ambriovi\'{c}-Ristov, Andreja and Benihoud, Karim and Majhen, Dragomira}, year = {2022}, pages = {9}, DOI = {10.1016/j.lfs.2021.120116}, chapter = {120116}, keywords = {Adenovirus vector, NGR retargeting peptide, CD13, Integrin}, journal = {Life sciences}, doi = {10.1016/j.lfs.2021.120116}, volume = {291}, issn = {0024-3205}, title = {Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency}, keyword = {Adenovirus vector, NGR retargeting peptide, CD13, Integrin}, chapternumber = {120116} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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