Naslov
Amiodarone and tamoxifen induced fatty liver injury and possible in vitro protective effect of GLP-1RA cotreatment

Autori
Omanović Kolarić, Tea ; Ninčević, Vjera ; Kizivat, Tomislav ; Kuna, Lucija ; Zjalić, Milorad ; Bilić- Ćurčć, Ines ; Vukadin, Sonja ; Roguljić, Hrvoje ; Včev, Aleksandar ; Smolić, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
8th European Congress of Pharmacology 2021 (EPHAR 2021)

Mjesto i datum
Prag, Češka Republika, 06.12.2021. - 08.12.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
fatty liver injury, GLP-1 receptor agonist, amiodarone, tamoxifen

Sažetak
Fatty liver disease is one of the most severe side effects of amiodarone and tamoxifen varying from simple steatosis to end-stage liver disease. In this study, we developed in vitro models of drug- induced fatty liver disease and analyzed the potential protective effect of glucagon-like- peptide-1 receptor agonist (GLP-1 RA). HuH7 cells were incubated with 5 µM to 20 µM of amiodarone, 1 µM to 10 µM of tamoxifen, and 1 nM to 100 nM of GLP-1 RA for various time periods (from 24h to a few days), respectively. Cell viability was measured by MTT assay. Changes in cell shape and the extent of hepatosteatosis were assessed by fluorescent microscopy. A significant decrease in cell viability occurred with tamoxifen administration in a dose-dependent manner after only 24h, whereas amiodarone did not diminish cell viability significantly in such a short period of time. Along with the decrease in cell viability, changes in cells appearance were also observed. Microsteatosis was demonstrated as the predominant form of injury. Co-treatment with GLP-1 RA enhanced cell viability, especially in tamoxifen- treated cells for almost 30% (p<0, 05), and this effect was dose-dependent. Further research is necessary in order to better elucidate the different effects of the abovementioned drugs in hepatic cell cultures.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA