Naslov
Aspergilli in damp dwellings - how diverse and dangerous are they?

Autori
Šegvić Klarić, Maja ; Jakšić, Daniela ; Kocsube, Sandor ; Kopjar, Nevenka ; Kifer, Domagoj ; Rašić, Dubravka ; Sulyok, Michael ; Šarkanj, Bojan ; Peraica, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of CROTOX 2021. U: Arhiv za higijenu rada i toksikologiju 72 (suppl.1) / Lyons, Daniel Mark ; Brčić Karačonji, Irena ; Kopjar, Nevenka - Zagreb : Hrvatsko toksikološko društvo, 2021, 19-19

Skup
6th Croatian congress of toxicology with international participation (CROTOX 2021)

Mjesto i datum
Rabac, Hrvatska, 04.10.2021. - 07.10.2021

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
5-methoxysterigmatocystin ; Aspergillus series Versicolores ; fungal metabolites ; genotoxicity ; sterigmatocystin

Sažetak
Aspergilli section Nidulantes series Versicolores, producers of the mycotoxin sterigmatocystin (STC), dominated the fungi isolated from air and dust in flood-affected homes (Gunja) and control houses (Gornji Stupnik) in Croatia. The majority of isolates were able to produce STC and some isolates also produced its derivate 5- methoxysterigmatocystin (5-MET-STC). How can this affect human health? Here we present: a) Aspergilli series Versicolores species diversity in air and dust (calmodulin sequence-based methods) ; b) occurrence of STC and metabolites from the STC biosynthesis pathway in dust (LC- MS/MS) ; and c) immunomodulatory and genotoxic effects of STC and 5-MET-STC in lungs of male Wistar rats upon intratracheal instillation in concentrations found in dust. Most of the Versicolores isolates were from Gunja (91/125). Most isolates belonging to A. jensenii, A. creber, A. puulaauensis, A. tennesseensis, A. venenatus produced both STC and 5-MET-STC. A. amoenus, A. fructus, A. griseoaurantiacus, A. pepii and A. protuberus produced STC but not 5-MET-STC, while A. sydowii did not produce any of these two toxins. Among the 75 fungal compounds detected in dust, metabolites from the STC pathway including STC, 5-MET-STC, veriscolorin C, averufin and averantin were among the most abundant metabolites, which prevailed in repaired houses in Gunja. The highest concentrations of STC (0.59 µg/g) and 5-MET-STC (7.70 µg/g) in dust were detected in Gunja. In rat lungs, the immunomodulatory effects of STC (0.4 µg/mL) and 5- MET-STC (5 µg/mL) were insignificant. The levels of DNA damage were similar although STC was applied at a 10-fold lower dose. The results of combined treatment suggested possible antagonistic interactions. This work was fully supported by the Croatian Science Foundation under the project MycotoxA (IP-09-2014-5982).

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA