Naslov
Features of dense fine speckled (DFS) pattern in everyday practice

Autori
Tešija Kuna, Andrea ; Vukasović, Ines ; Bunjevac, Amalija ; Herceg, Ivona ; Đerek, Lovorka ; Njegovan, Milena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Autoimmunity 2021, Athens: 12th International Congress on Autoimmunity / - : Kenes Group, 2021

Skup
12th International Congress on Autoimmunity

Mjesto i datum
Atena, Grčka, 28.05.2021. - 01.06.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Diagnostic measurement ; Dense fine speckled (DFS) pattern ; Anti-DFS70 reactivity
(diagnostic measurement ; dense fine speckled (DFS) pattern ; anti-DFS70 reactivity)

Sažetak
Background: Nucleardense fine speckled (DFS) patternon Hep-2 substrate with confirmed DFS70 reactivity, as the single ANA specificity, has been suggested as exclusion marker for systemic autoimmunerheumatic diseases (SARD). The aim was to asses:the frequencyof DFS pattern, the rate of DFS pattern with confirmed DFS70 reactivity, the association between DFS70 positivity and ANA titer. Materials and methods: We examined the resultsof ANA IIF in periodof 8.5 months. ANA IIF was performed using Hep-2 test system (Euroimmun, Luebeck, Germany). According to internal protocol, anti-DFS70 is performed only in the DFS pattern positive patients with requested ENA in whom ENA7 screen test is negative. ENA7 and anti-DFS70 was determined using Quanta Flash chemmiluminescenceimmunoassays on BIO-FLASH instrument (all INOVA Diagnostics, Inc., San Diego, CA, USA). Results: Positive ANA was found in 1734/4473 (38.7%) and DFS pattern in 296/1734 (17.1%) samples.Following internal protocol, anti- DFS70 reactivity was determined for 180/296 samples and confirmed in 55/180 (30, 5%) samples. Frequency of positive anti-DFS70 was compared for 4 ANA titer categories(1/160, 1/320, 1/640, >1/640). Significant difference was found (P = 0, 0009) with trend of rising frequency in paralel with rising ANA titer (12.8%, 24.6%, 38.1%, 52.6%, respectively).Low correlation was found between anti-DFS level and ANA titer (p= 0.295 (95%CI 0.155-0.423)P=0.0001). Conclusion: Anti-DFS70 reactivity was confirmed in 30.5% DFS pattern ssuggesting that the rest can be referred to pseudo-DFS pattern which seems to be more frequent. Our results confirmed the association of DFS70 reactivity with high ANA titer.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija

Napomena
Kongres je održan u virtualnom okruženju. Knjiga
sažetaka objavljena na CD mediju.

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