Naslov
Comparison of five different reagents for anticardiolipin antibodies determination

Autori
Đerek, Lovorka ; Drvar, Vedrana ; Stančin, Nevenka ; Njegovan, Milena ; Vukasović, Ines ; Tešija Kuna, Andrea

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Autoimmunity 2021, Athens: 12th International Congress on Autoimmunity / - : Kenes Group, 2021

Skup
12th International Congress on Autoimmunity

Mjesto i datum
Atena, Grčka, 28.05.2021. - 01.06.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Anticardiolipin antibodies ; Antiphospholipid syndrome ; Comparison of methods
(anticardiolipin antibodies ; antiphospholipid syndrome ; comparison of methods)

Sažetak
Background and aims AnticardiolipinsIgG (aCL-IgG) and IgM (aCL-IgM) antibodies are used asone of the criteria forantiphospholipid syndrome (APS). Our aim was to compare the results of five different methods for anticardiolipinantibodiesto obtain an insight into method comparability. Methods The study included 38 patients (35 women and 3 men) with history or suspicion of thrombotic event. aCL-IgG and aCL-IgM were measured using 5 different reagents: Anti-CardiolipinELISA on Analyzer I-2P (all EUROIMMUN AG, Lubeck, Germany), CardiolipinIgGandIgMchemiluminiscentimmunoassay (CLIA) on IDS iSYS (all Immunodiagnostic Systems Limited, Boldon, United Kingdom), QUANTA Flash® aCLCLIA on BIO-FLASH® (all Inova Diagnostics Inc, San Diego, USA), EliACardiolipinfluoroenzymeimmunoassay on Phadia 100 (all ThermoFisherDiagnosticsGmbHFreiburg, Germany) and Anti-CardiolipinIgG/IgM ELISA (Orgentec, Mainz, Germany). Results were categorized as positive or negative and interclass correlation coefficient was calculated (MedCalc version 14.8.1) Results Interclass correlation coefficient showed very good degree of consistency inaverage (interclass correlation:aCL-IgG 0, 7789(95%CI:0, 6442-0, 8732) and aCL-IgM 0, 8594(95%CI:0, 2659-0, 5793)) but poor or moderate reliability on single ratings (interclass correlation:aCL- IgG0, 4133(95%CI:0, 2659-0, 5793) andaCL-IgM 0, 5501(95%CI:0, 4063-0, 6952)). Conclusions Our results showed a poor comparability betweenaCLmethods which indicates a lack of standardization. Considering our results we give rise to the fact thatit is possibleto obtain different information from one aCL result for the same patient. The importance of simultaneous measurement of all antibodies included in the APS criteria should be emphasized in order to obtain clinically significant information.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija

Napomena
Kongres je održan u virtualnom okruženju. Knjiga
sažetaka objavljena na CD mediju.

POVEZANOST RADA