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Pregled bibliografske jedinice broj: 1167889

Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison

Franco, S.; Cougoule, N.; Tison, A.; Del Cont, A.; Gastaldi, C.; ILC Consortium (...I., Tlak Gajger; ...); Duquesne, V.
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison // Insects, 13 (2022), 33, 20 doi:10.3390/insects13010033 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1167889 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison

Autori
Franco, S. ; Cougoule, N. ; Tison, A. ; Del Cont, A. ; Gastaldi, C. ; ILC Consortium (...I., Tlak Gajger ; ...) ; Duquesne, V.

Izvornik
Insects (2075-4450) 13 (2022); 33, 20

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diagnosis ; inter-laboratory comparison ; morphology ; real-time PCR ; honey bee ; Aethina tumida ; Small Hive Beetle

Sažetak
The Small Hive Beetle (Aethina tumida Murray, 1867) is an invasive scavenger of honeybees. Originally endemic in sub-Saharan Africa, it is regulated internationally in order to preserve the areas still free from this species. To ensure the reliability of official diagnoses in case of introduction, an inter-laboratory comparison was organised on the identification of A. tumida by morphology and real- time PCR. Twenty-two National Reference Laboratories in Europe participated in the study and analysed 12 samples with adult coleopterans and insect larvae. The performance of the laboratories was evaluated in terms of sensitivity and specificity. Sensitivity was satisfactory for all the participants and both types of methods, thus fully meeting the diagnostic challenge of confirming all truly positive cases as positive. Two participants encountered specificity problems. For one, the anomaly was minor whereas, for the other, the issues concerned a larger number of results, especially real-time PCR, which probably were related to inexperience with this technique. The comparison demonstrated the reliability of official diagnosis, including the entire analytical process of A. tumida identification: from the first step of the analysis to the expression of opinions. The performed diagnostic tools, in parallel with field surveillance, are essential to managing A. tumida introduction.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
Ivana Tlak Gajger je član ILC Consortium.



POVEZANOST RADA

Ustanove:
Veterinarski fakultet, Zagreb

Profili:

Avatar Url Ivana Tlak Gajger (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Franco, S.; Cougoule, N.; Tison, A.; Del Cont, A.; Gastaldi, C.; ILC Consortium (...I., Tlak Gajger; ...); Duquesne, V.
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison // Insects, 13 (2022), 33, 20 doi:10.3390/insects13010033 (međunarodna recenzija, članak, znanstveni)
Franco, S., Cougoule, N., Tison, A., Del Cont, A., Gastaldi, C., ILC Consortium (...I., T., ...) & Duquesne, V. (2022) Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison. Insects, 13, 33, 20 doi:10.3390/insects13010033.
@article{article, author = {Franco, S. and Cougoule, N. and Tison, A. and Del Cont, A. and Gastaldi, C. and ILC Consortium (...I., Tlak Gajger and Duquesne, V.}, year = {2022}, pages = {20}, DOI = {10.3390/insects13010033}, chapter = {33}, keywords = {diagnosis, inter-laboratory comparison, morphology, real-time PCR, honey bee, Aethina tumida, Small Hive Beetle}, journal = {Insects}, doi = {10.3390/insects13010033}, volume = {13}, issn = {2075-4450}, title = {Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison}, keyword = {diagnosis, inter-laboratory comparison, morphology, real-time PCR, honey bee, Aethina tumida, Small Hive Beetle}, chapternumber = {33} }
@article{article, author = {Franco, S. and Cougoule, N. and Tison, A. and Del Cont, A. and Gastaldi, C. and ILC Consortium (...I., Tlak Gajger and Duquesne, V.}, year = {2022}, pages = {20}, DOI = {10.3390/insects13010033}, chapter = {33}, keywords = {diagnosis, inter-laboratory comparison, morphology, real-time PCR, honey bee, Aethina tumida, Small Hive Beetle}, journal = {Insects}, doi = {10.3390/insects13010033}, volume = {13}, issn = {2075-4450}, title = {Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison}, keyword = {diagnosis, inter-laboratory comparison, morphology, real-time PCR, honey bee, Aethina tumida, Small Hive Beetle}, chapternumber = {33} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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