Pregled bibliografske jedinice broj: 1163290
Interaction of Spin-Labeled HPMA-based Nanoparticles with Human Blood Plasma Proteins
Interaction of Spin-Labeled HPMA-based Nanoparticles with Human Blood Plasma Proteins // Book of Abstracts, 4th European Conference on Smart Inorganic Polymers / Valić, Srećko ; Tota, Marin ; Klepac, Damir (ur.).
Zagreb: Institut Ruđer Bošković, 2018. str. 45-45 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1163290 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Interaction of Spin-Labeled HPMA-based Nanoparticles with Human Blood Plasma Proteins
Autori
Klepac, Damir ; Kostková, Hana ; Petrova, Svetlana ; Chytil, Petr ; Etrych, Tomáš ; Kereïche, Sami ; Raška, Ivan ; Weitz, David A. ; Filippov, Sergey, K.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts, 4th European Conference on Smart Inorganic Polymers
/ Valić, Srećko ; Tota, Marin ; Klepac, Damir - Zagreb : Institut Ruđer Bošković, 2018, 45-45
ISBN
978-953-7941-20-8
Skup
4th European Conference on Smart Inorganic Polymers
Mjesto i datum
Zagreb, Hrvatska, 01.03.2018. - 03.03.2018
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
HPMA copolymers ; drug delivery ; blood plasma ; proteins ; EPR/ESR ; light scattering ; Cryo-TEM
Sažetak
We revised current understanding of the protein corona that is created on the surface of the nanoparticles in blood plasma after intravenous injection. These nanoparticles are based on N-(2-hydroxypropyl)methacrylamide (HPMA) and have been tested both in vitro and in vivo. We studied the interaction of nanoparticles with blood plasma and selected blood plasma proteins by electron paramagnetic resonance (EPR), isothermal titration calorimetry (ITC), dynamic light scattering (DLS), and cryo transmission electron microscopy (cryo-TEM). The copolymers were labeled with TEMPO radicals to monitor changes in polymer chain dynamics caused by protein adsorption. It was found that HSA, LDL, HDL, IgG and plasma have very low binding affinity to nanoparticles. In contrast with the “hard-soft coronas” paradigm used to describe a double shell layer of proteins, we observed no hard corona around HPMA-based nanoparticles. Our study suggests that a classical “hard corona-soft corona” paradigm is not valid for all types of nanoparticles but rather represents an extreme case for some types of charged and hydrophobic nanoparticles.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Kemijsko inženjerstvo, Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
NadSve-Sveučilište u Rijeci-uniri-prirod-18-299 - Sinteza i karakterizacija pametnih polimernih nanočestica s nitroksidnim radikalima (Klepac, Damir, NadSve - 2018) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Damir Klepac
(autor)
