Pregled bibliografske jedinice broj: 1163243
Magnesium ions reversibly bind to DNA double stranded helix in thin films
Magnesium ions reversibly bind to DNA double stranded helix in thin films // Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 268 (2021), 120663, 10 doi:10.1016/j.saa.2021.120663 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1163243 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Magnesium ions reversibly bind to DNA double stranded helix in thin films
Autori
Serec, Kristina ; Dolanski Babić, Sanja ; Tomić, Silvia
Izvornik
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (1386-1425) 268
(2021);
120663, 10
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
DNA thin films ; FTIR spectroscopy ; Mg2+ ; ion depletion ; asymmetric and symmetric PO2 strecthing modes ; band shape analysis of phosphate group vibrations
Sažetak
Effects of magnesium (Mg2+) ions on the stability and structural properties of double-stranded DNA are vitally important for DNA folding and functional behavior. Complementing our previous study on highly hydrated thin films of DNA with sodium counterions, with no buffer (pH ≈ 6) and surrounded with Mg2+ cations, here we use Fourier transform infrared spectroscopy and band shape analysis to explore in detail the vibrational signatures of DNA-magnesium interaction in the case when DNA charges are neutralized solely by Mg2+ cations, hereafter called MgDNA. Ion atmosphere has been controlled by the magnesium to phosphate molar concentration ratio r which varied between 0.0067 and 10. For r = 0 we find that spectral features in the base region remain similar as in DNA, whereas changes in the backbone region indicate that the B conformation becomes fully stabilized. With increasing r a pronounced structural reshaping occurs in the phosphate backbone region indicating a blue shift of the asymmetric band, while the symmetric band does not show any displacement in frequency. The band shape analysis of overlapping peaks in the respective phosphate regions demonstrates that the number of constituent modes as well as their positions in frequency do not change, whereas their intensities and bandwidths display disparate changes. The results reflect a variety of local environments at the DNA backbone due to a heterogeneous ion atmosphere with randomly distributed magnesium ions and local patterns of hydrogen bonds which change with increasing r. Remarkably, after crowded r =10 ion atmosphere is depleted, Mg induced spectral changes vanish and structural features of MgDNA (r ≈ 0) are fully restored. Overall results strongly suggest that in MgDNA on highly hydrated thin films the hydrogen-base pairing remains preserved and that Mg2+ ions, similar to sodium ions, retain their mobility and interact with double helix via water-mediated electrostatic forces.
Izvorni jezik
Engleski
Znanstvena područja
Fizika, Kemija, Biologija, Temeljne medicinske znanosti
POVEZANOST RADA
Ustanove:
Institut za fiziku, Zagreb,
Medicinski fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE