Pregled bibliografske jedinice broj: 1163160
Oleanolic acid induces mitophagy and chemosenzitises human colon carcinoma cells
Oleanolic acid induces mitophagy and chemosenzitises human colon carcinoma cells // Biochemical Insights into Molecular Mechanisms / Spasojević, Ivan - Kragujevac : Faculty of Chemistry, Serbian Biochemical Society, 2021 / Spasojević, Ivan (ur.).
Kragujevac, Srbija: Faculty of Chemistry, Serbian Biochemical Society, Colorgrafx, Belgrade, 2021. str. 177-177 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1163160 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Oleanolic acid induces mitophagy and chemosenzitises
human colon carcinoma cells
Autori
Potočnjak, Iva ; Šimić, Lidija ; Vukelić, Iva ; Batičić, Lara ; Domitrović, Robert
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Biochemical Insights into Molecular Mechanisms / Spasojević, Ivan - Kragujevac : Faculty of Chemistry, Serbian Biochemical Society, 2021
/ Spasojević, Ivan - : Faculty of Chemistry, Serbian Biochemical Society, Colorgrafx, Belgrade, 2021, 177-177
Skup
10th Serbian Biochemical Society Tenth Conference with international participation: Biochemical Insights into Molecular Mechanisms
Mjesto i datum
Kragujevac, Srbija, 24.09.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
oleanolic acid ; colon cancer ; apoptosis ; autophagy ; mitophagy, 5-fluorouracil
Sažetak
Oleanolic acid (OA) has been shown to possess numerous beneficial health effects. However, the effect of OA on mitophagy in colon cancer cells is unknown. The current study aimed to investigate the mechanism of autophagy/mitophagy in the anticancer activity of OA in HCT116 human colon carcinoma cells. OA dose-dependently reduced viability of HCT116 cells, with IC50 29.8 μΜ. The expression of cleaved caspase-3 and PARP1 increased after OA treatment, suggesting that OA induces apoptosis in HCT116 cells. Concurrently, OA induced autophagy in cancer cells, evidenced by the increase in expression of Beclin-1, Atg5 and LC3B-II, which played a prosurvival role. The induction of mitophagy was suggested by increased expression of p62 and PINK1 and reduced expression of TOMM20, which colocalized with LC3B. OA also induced nuclear accumulation of FOXO3a and p- FOXO3a. The cytotoxic activity of OA coincided with downregulation of the PI3K/Akt and ERK1/2 pathways and activation of AMPK, JNK1 and p38. In addition, OA enhanced the cytotoxicity of 5-FU. Our results showed concomitant induction of apoptosis and survival autophagy/mitophagy in HCT116 cells by OA via modulation of key signaling pathways involved in tumorigenesis. Additionally, we showed chemosensitization of HCT116 cells to 5- FU by OA.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Farmacija
POVEZANOST RADA
Projekti:
NadSve-Sveučilište u Rijeci-uniri-mladi-biomed-20-17 - Modulacija MEK-ERK MAPK signalnog puta u CP-induciranoj mitofagiji u bubrezima (Potočnjak, Iva, NadSve ) ( CroRIS)
MEDRI--uniri-biomed-18-30 - Interakcija lijekova i fitokemikalija in vitro i in vivo: uloga FOXO signalnog puta (Domitrović, Robert, MEDRI ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Lara Batičić
(autor)
Iva Potočnjak
(autor)
Lidija Šimić
(autor)
Iva Vukelić
(autor)
Robert Domitrović
(autor)