Naslov
Development of a novel dispersive liquid-liquid microextraction method for the simultaneous analysis of six anticancer drugs in human plasma
(Razvoj nove metode disperzivne mikroekstrakcije tekuće-tekuće za istovremenu analizu šest antitumorskih lijekova u ljudskoj plazmi)

Autori
Turković, Lu ; Koraj, Natan ; Silovski, Tajana ; Ščavničar, Andrijana ; Lovrić, Mila ; Nigović, Biljana ; Sertić, Miranda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27th Croatian Meeting of Chemists and Chemical Engineers, Book of Abstracts / Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna - Zagreb : Hrvatsko kemijsko društvo, 2021, 224-224

Skup
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI)

Mjesto i datum
Veli Lošinj, Hrvatska, 05.10.2021. - 08.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oncology ; bioanalysis ; microextraction ; therapeutic drug monitoring

Sažetak
Palbociclib, ribociclib and abemaciclib are novel anticancer agents used in combination with anastrozole, letrozole or fulvestrant in breast cancer treatment. They significantly improve therapeutic outcomes, but still exhibit an array of side-effects and a potential for inter- individual variabilities, so therapeutic drug monitoring (TDM) could prove beneficial [1]. A suitable bioanalytical method therefore needs to be developed, encompassing both the sample preparation procedure and the analytical system parameters. Optimal sample preparation ensures maximal analyte extraction recoveries, while minimizing matrix effect and prolonging the instrument lifetime [2]. Dispersive liquid-liquid microextraction is a technique in which a mixture of an organic extractant and disperser solvents is quickly injected into an aqueous solution, forming an emulsion that facilitates fast and efficient extraction. In biological sample preparation, protein precipitation (PPT) usually precedes the extraction. Since acetonitrile is commonly used as both a PPT agent and a disperser solvent, the supernatant after PPT can immediately be mixed with an extractant solvent and injected into an aqueous solution [3]. In this work, various conditions affecting analytes’ extraction recoveries were investigated: type and volume of the extractant and disperser solvent, aqueous phase pH, addition of salt, and ultrasonication. In optimal conditions, for 50 µL of plasma sample, 200 µL of acetonitrile is added for PPT. 200 µL of the supernatant is withdrawn, mixed with 400 µL of chloroform and injected into 100 µL of 5 % w/V aqueous sodium citrate solution. The organic layer is evaporated to dryness, reconstituted in 40 µL 65 % V/V methanol and analysed by liquid chromatography. The proposed method is fast, simple, with low sample and solvent consumption, and offers very high extraction yields (87.2 to 97.8 %) with significantly less matrix interferences compared to PPT alone.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA