Pregled bibliografske jedinice broj: 1162798
Effects of uroguanylin’s signaling pathways on ischemic lesion size
Effects of uroguanylin’s signaling pathways on ischemic lesion size // Virtual FENS regional meeting 2021 : book of abstracts
Krakov, 2021. str. 247-248 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1162798 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Effects of uroguanylin’s signaling pathways on
ischemic lesion size
Autori
Ratko, Martina ; Habek, Nikola ; Dobrivojević Radmilović, Marina ; Škokić, Siniša ; Justić, Helena ; Barić, Anja ; Dugandžić, Aleksandra
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Virtual FENS regional meeting 2021 : book of abstracts
/ - Krakov, 2021, 247-248
Skup
FENS regional meeting (FRM 2021)
Mjesto i datum
Online, 25.08.2021. - 27.08.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
guanylate cyclase-C ; middle cerebral artery occlusion ; MR imaging ; Ca2+ signaling
Sažetak
Stroke is one of the leading causes of mortality and disability in industrialized countries. Guanylate cyclase (GC) A activation has a neuroprotective effect after ischemic stroke [1] therefore the aim of this study is to determine if agonists of GC-C have similar effects. Uroguanylin (UGN) activates guanylate cyclase C (GC-C) and a Ca2+-dependent signaling pathway [2]. In this study, middle cerebral artery occlusion (MCAO) was performed on wild type (WT), GC-C KO and UGN KO mice. Before and 24h after MCAO MR images were taken. 48h following MCAO brain slices were isolated and Ca2+ response to UGN stimulation was recorded. Immunohistochemical staining was performed with GC-C, NeuN, and GFAP antibodies. WT and UGN KO animals exhibit a stronger Ca2+ response to UGN stimulation in astrocytes of the ischemic penumbra in cerebral cortex but not in the unaffected hemisphere. This stronger activation is gone in GC-C KO animals which results in development of smaller ischemic le¬sions in GC-C KO mice compared to their WT littermates. Considering the fact that GC-C becomes expressed on penumbral astrocytes following ischemia, while in normoxic conditions it is expressed only in cortical neurons, effects of GC-C on intracellular Ca2+ concentration could be due to activation of cGMP-dependent Ca2+ channels in penumbral astrocytes [3]. Stronger activation of the Ca2+-dependent signaling pathway could lead to the development of larger ischemic lesions, possibly through upregula¬tion of Na+/H+ exchanger, tissue acidification, and neuronal death. [1] Dobrivojević M, Špiranec K, Gorup D, Erjavec I, Habek N, Radmilović M, Unfirer S, Ćosić A, Drenjančević I, Gajović S, Sinđić A (2016) Urodilatin reverses the detrimental influence of bradykinin in acute ischemic stroke. Exp Neurol 284:1-10. [2] Habek N, Ratko M, Dugandžić A (2021) Uroguanylin Increases Ca2+ Concentration in Astrocytes via Guanylate Cyclase C-independent Signalling Pathway. Croat Med J. In press. [3] Rappaport JA, Waldman SA (2018) The Guanylate Cyclase C-cGMP Signaling Axis Opposes Intestinal Epithe¬lial Injury and Neoplasia. Front Oncol 8:299. Funding: Research was funded by the Scientific Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic- ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund). The work of doctoral student Anja Barić has been fully supported by the “Young researchers’ career development project – training of doctoral students” and project BRADISCHEMIA (UIP-2017-05- 8082) of the Croatian Science Foundation funded by the European Union from the European Social Fund.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (ZCI Neuro) (Judaš, Miloš) ( CroRIS)
--UIP-2017-05-8082 - Uloga bradikinina u ishemiji mozga i mrežnice u mišjim modelima dijabetesa (BRADISCHEMIA) (Radmilović Dobrivojević, Marina) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Martina Ratko
(autor)
Aleksandra Sinđić
(autor)
Siniša Škokić
(autor)
Nikola Habek
(autor)
Anja Barić
(autor)