Pregled bibliografske jedinice broj: 1162795
Effect of uroguanylin on development of ischemic brain lesion
Effect of uroguanylin on development of ischemic brain lesion // 8th Croatian neuroscience congress : book of abstracts
Zagreb, 2021. str. 98-98 (poster, domaća recenzija, sažetak, stručni)
CROSBI ID: 1162795 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Effect of uroguanylin on development of ischemic
brain lesion
Autori
Ratko, Martina ; Habek, Nikola ; Dobrivojević Radmilović, Marina ; Škokić, Siniša ; Justić, Helena ; Barić, Anja ; Dugandžić, Aleksandra
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni
Izvornik
8th Croatian neuroscience congress : book of abstracts
/ - Zagreb, 2021, 98-98
Skup
8th Croatian neuroscience congress
Mjesto i datum
Online, 24.09.2021. - 25.09.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
guanylate cyclase-C ; middle cerebral artery occlusion ; MR imaging ; Ca2+ signaling
Sažetak
Stroke is one of the leading causes of mortality and disability in industrialized countries. Guanylate cyclase (GC) A activation has a neuroprotective effect after ischemic stroke therefore the aim of this study is to determine if agonists of GC-C have similar effects. Uroguanylin (UGN) activates guanylate cyclase C (GC-C) and a Ca2+ -dependent signaling pathway. In this study, middle cerebral artery occlusion (MCAO) was performed on wild type (WT), GC-C KO and UGN KO mice. Before and 24h after MCAO MR images were taken. 48h following MCAO brain slices were isolated and Ca2+ response to UGN stimulation was recorded. Immunohistochemical staining was performed with GC-C, NeuN, and GFAP antibodies. WT and UGN KO animals exhibit a stronger Ca2+ response to UGN stimulation in astrocytes of the ischemic penumbra in cerebral cortex but not in the unaffected hemisphere. This stronger activation is gone in GC-C KO animals which results in development of smaller ischemic lesions in GC-C KO mice compared to their WT littermates. Considering the fact that GC-C becomes expressed on penumbral astrocytes following ischemia, while in normoxic conditions it is expressed only in cortical neurons, effects of GC-C on intracellular Ca2+ concentration could be due to activation of cGMP-dependent Ca2+ channels in penumbral astrocytes. Stronger activation of the Ca2+- dependent signaling pathway could lead to the development of larger ischemic lesions in WT compared to GC-C KO animals, possibly through upregulation of Na+/H+ exchanger followed by tissue acidification and neuronal death. Acknowledgements: Research was funded by the Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic-ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund). The work of doctoral student Anja Barić has been fully supported by the “Young researchers' career development project – training of doctoral students” and project BRADISCHEMIA (UIP-2017-05- 8082) of the Croatian Science Foundation funded by the European Union from the European Social Fund.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (ZCI Neuro) (Judaš, Miloš) ( CroRIS)
--UIP-2017-05-8082 - Uloga bradikinina u ishemiji mozga i mrežnice u mišjim modelima dijabetesa (BRADISCHEMIA) (Radmilović Dobrivojević, Marina) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Martina Ratko
(autor)
Aleksandra Sinđić
(autor)
Siniša Škokić
(autor)
Nikola Habek
(autor)
Anja Barić
(autor)