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Pregled bibliografske jedinice broj: 1162795

Effect of uroguanylin on development of ischemic brain lesion


Ratko, Martina; Habek, Nikola; Dobrivojević Radmilović, Marina; Škokić, Siniša; Justić, Helena; Barić, Anja; Dugandžić, Aleksandra
Effect of uroguanylin on development of ischemic brain lesion // 8th Croatian neuroscience congress : book of abstracts
Zagreb, 2021. str. 98-98 (poster, domaća recenzija, sažetak, stručni)


CROSBI ID: 1162795 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Effect of uroguanylin on development of ischemic brain lesion

Autori
Ratko, Martina ; Habek, Nikola ; Dobrivojević Radmilović, Marina ; Škokić, Siniša ; Justić, Helena ; Barić, Anja ; Dugandžić, Aleksandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
8th Croatian neuroscience congress : book of abstracts / - Zagreb, 2021, 98-98

Skup
8th Croatian neuroscience congress

Mjesto i datum
Online, 24.09.2021. - 25.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
guanylate cyclase-C ; middle cerebral artery occlusion ; MR imaging ; Ca2+ signaling

Sažetak
Stroke is one of the leading causes of mortality and disability in industrialized countries. Guanylate cyclase (GC) A activation has a neuroprotective effect after ischemic stroke therefore the aim of this study is to determine if agonists of GC-C have similar effects. Uroguanylin (UGN) activates guanylate cyclase C (GC-C) and a Ca2+ -dependent signaling pathway. In this study, middle cerebral artery occlusion (MCAO) was performed on wild type (WT), GC-C KO and UGN KO mice. Before and 24h after MCAO MR images were taken. 48h following MCAO brain slices were isolated and Ca2+ response to UGN stimulation was recorded. Immunohistochemical staining was performed with GC-C, NeuN, and GFAP antibodies. WT and UGN KO animals exhibit a stronger Ca2+ response to UGN stimulation in astrocytes of the ischemic penumbra in cerebral cortex but not in the unaffected hemisphere. This stronger activation is gone in GC-C KO animals which results in development of smaller ischemic lesions in GC-C KO mice compared to their WT littermates. Considering the fact that GC-C becomes expressed on penumbral astrocytes following ischemia, while in normoxic conditions it is expressed only in cortical neurons, effects of GC-C on intracellular Ca2+ concentration could be due to activation of cGMP-dependent Ca2+ channels in penumbral astrocytes. Stronger activation of the Ca2+- dependent signaling pathway could lead to the development of larger ischemic lesions in WT compared to GC-C KO animals, possibly through upregulation of Na+/H+ exchanger followed by tissue acidification and neuronal death. Acknowledgements: Research was funded by the Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic-ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund). The work of doctoral student Anja Barić has been fully supported by the “Young researchers' career development project – training of doctoral students” and project BRADISCHEMIA (UIP-2017-05- 8082) of the Croatian Science Foundation funded by the European Union from the European Social Fund.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
--KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (ZCI Neuro) (Judaš, Miloš) ( CroRIS)
--UIP-2017-05-8082 - Uloga bradikinina u ishemiji mozga i mrežnice u mišjim modelima dijabetesa (BRADISCHEMIA) (Radmilović Dobrivojević, Marina) ( CroRIS)

Ustanove:
Medicinski fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

www.hdn.hr

Citiraj ovu publikaciju:

Ratko, Martina; Habek, Nikola; Dobrivojević Radmilović, Marina; Škokić, Siniša; Justić, Helena; Barić, Anja; Dugandžić, Aleksandra
Effect of uroguanylin on development of ischemic brain lesion // 8th Croatian neuroscience congress : book of abstracts
Zagreb, 2021. str. 98-98 (poster, domaća recenzija, sažetak, stručni)
Ratko, M., Habek, N., Dobrivojević Radmilović, M., Škokić, S., Justić, H., Barić, A. & Dugandžić, A. (2021) Effect of uroguanylin on development of ischemic brain lesion. U: 8th Croatian neuroscience congress : book of abstracts.
@article{article, author = {Ratko, Martina and Habek, Nikola and Dobrivojevi\'{c} Radmilovi\'{c}, Marina and \v{S}koki\'{c}, Sini\v{s}a and Justi\'{c}, Helena and Bari\'{c}, Anja and Dugand\v{z}i\'{c}, Aleksandra}, year = {2021}, pages = {98-98}, keywords = {guanylate cyclase-C, middle cerebral artery occlusion, MR imaging, Ca2+ signaling}, title = {Effect of uroguanylin on development of ischemic brain lesion}, keyword = {guanylate cyclase-C, middle cerebral artery occlusion, MR imaging, Ca2+ signaling}, publisherplace = {online} }
@article{article, author = {Ratko, Martina and Habek, Nikola and Dobrivojevi\'{c} Radmilovi\'{c}, Marina and \v{S}koki\'{c}, Sini\v{s}a and Justi\'{c}, Helena and Bari\'{c}, Anja and Dugand\v{z}i\'{c}, Aleksandra}, year = {2021}, pages = {98-98}, keywords = {guanylate cyclase-C, middle cerebral artery occlusion, MR imaging, Ca2+ signaling}, title = {Effect of uroguanylin on development of ischemic brain lesion}, keyword = {guanylate cyclase-C, middle cerebral artery occlusion, MR imaging, Ca2+ signaling}, publisherplace = {online} }




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