Pregled bibliografske jedinice broj: 1162793
Effects of uroguanylin’s signaling pathways on schemic lesion size
Effects of uroguanylin’s signaling pathways on schemic lesion size // Mind & brain : abstract book 2021
Pula, 2021. str. 86-86 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 1162793 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Effects of uroguanylin’s signaling pathways on
schemic lesion size
Autori
Ratko, Martina ; Habek, Nikola, Dobrivojević Radmilović, Marina ; Škokić, Siniša ; Justić, Helena ; Barić, Anja ; Dugandžić, Aleksandra
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Mind & brain : abstract book 2021
/ - Pula, 2021, 86-86
Skup
60th International neuropsychiatric congress Mind & brain
Mjesto i datum
Online, 27.05.2021. - 30.05.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
guanylate cyclase-C ; middle cerebral artery occlusion ; MR imaging ; Ca2+ signaling
Sažetak
Introduction/Objectives: Stroke is one of the leading causes of mortality and disability in industrialized countries. Guanylate cyclase (GC) A activation has a neuroprotective effect after ischemic stroke. Therefore, the aim of this study is to determine if agonists of GC-C have similar effects. Uroguanylin (UGN) activates guanylate cyclase C (GC-C) and a Ca2+-dependent signaling pathway. Participants, Materials/Methods: In this study, middle cerebral artery occlusion (MCAO) was performed on wild type (WT), GC-C KO and UGN KO mice. Before and 24h after MCAO MR images were taken. 48h following MCAO brain slices were isolated and Ca2+ response to UGN stimulation was recorded. Immunohistochemical staining was performed with GC-C, NeuN, and GFAP antibodies. Results: WT and UGN KO animals exhibit a stronger Ca2+ response to UGN stimulation in astrocytes of the ischemic penumbra in cerebral cortex but not in the unaffected hemisphere. This stronger activation is gone in GC-C KO animals which results in development of smaller ischemic lesions in GC-C KO mice compared to their WT littermates. Considering the fact that GC-C becomes expressed on penumbral astrocytes following ischemia, while in normoxic conditions it is expressed only in cortical neurons, effects of GC-C on intracellular Ca2+ concentration could be due to activation of cGMP-dependent Ca2+ channels in penumbral astrocytes. Conclusions: Stronger activation of the Ca2+-dependent signaling pathway could lead to the development of larger ischemic lesions, possibly through upregulation of Na+/H+ exchanger, tissue acidification, and neuronal death.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (ZCI Neuro) (Judaš, Miloš) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Martina Ratko
(autor)
Aleksandra Sinđić
(autor)
Siniša Škokić
(autor)
Nikola Habek
(autor)
Anja Barić
(autor)