Pregled bibliografske jedinice broj: 1162781
The role of uroguanylin’s GC-C independent signalling pathway in the development of ischemic stroke
The role of uroguanylin’s GC-C independent signalling pathway in the development of ischemic stroke // 14th Annual Meeting of Croatian Physiological Society with International Participation
Hrvatska; online, 2020. str. 18-18 (predavanje, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 1162781 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The role of uroguanylin’s GC-C independent signalling
pathway in the development of ischemic stroke
Autori
Ratko, Martina ; Habek, Nikola ; Dobrivojević Radmilović, Marina ; Škokić, Siniša ; Justić, Helena ; Barić, Anja ; Dugandžić, Aleksandra
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
14th Annual Meeting of Croatian Physiological Society with International Participation
Mjesto i datum
Hrvatska; online, 25.09.2020. - 26.09.2020
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
middle cerebral artery occlusion, MR imaging, Ca2+ signaling
Sažetak
INTRODUCTION: Recent research has shown that the activation of guanylate cyclase (GC)-A, but not guanylate cyclase B (GC-B), leads to a decrease of brain lesion size following middle cerebral artery occlusion (MCAO). AIM: Our aim was to investigate the potential role of uroguanylin (UGN)-dependent signalling pathways (GC-C or Ca2+-dependent) in the development of ischemic stroke. METHODS: Lesion volumes were measured in WT, GC-C and UGN KO mice by MR imaging 1 day after MCAO. Intracellular Ca2+ concentrations were measured in slices of ipsilateral and contralateral hemispheres. RESULTS: GC-C KO animals exhibited significantly smaller lesion volumes 1 day after MCAO than WT mice. This difference was not present between UGN KO and WT littermates. The hemisphere with stroke shows a greater increase in intracellular Ca2+ concentrations following UGN stimulation compared to the healthy hemisphere of the same animal. This difference is absent in GC-C KO animals, while UGN KO animals exhibit a prolonged Ca2+ response compared to their WT littermates. CONCLUSION: The reduction in stroke volume present in GC-C KO but not UGN KO mice indicates that the activation of the Ca2+-dependent signalling pathway may have potentially harming effects. The possible mechanism could be increased UGN activity on pH regulation in astrocytes via Ca2+ signalling pathway and worsening of existing interstitial post ischemic acidosis. ACKNOWLEDGEMENTS Research was funded by the Scientific Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic-ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund). The work of doctoral student Anja Barić has been fully supported by the “Young researchers' career development project – training of doctoral students” and project BRADISCHEMIA (UIP-2017-05-8082) of the Croatian Science Foundation funded by the European Union from the European Social Fund.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (ZCI Neuro) (Judaš, Miloš) ( CroRIS)
--UIP-2017-05-8082 - Uloga bradikinina u ishemiji mozga i mrežnice u mišjim modelima dijabetesa (BRADISCHEMIA) (Radmilović Dobrivojević, Marina) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Martina Ratko
(autor)
Aleksandra Sinđić
(autor)
Siniša Škokić
(autor)
Nikola Habek
(autor)
Anja Barić
(autor)