Pregled bibliografske jedinice broj: 1162777
The role of uroguanylin in ischemic stroke
The role of uroguanylin in ischemic stroke // 12th FENS Forum of Neuroscience
Virtualni kongres (Glasgow, Ujedinjeno Kraljevstvo), 2020. 3767, 1 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1162777 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The role of uroguanylin in ischemic stroke
Autori
Ratko, Martina ; Habek, Nikola ; Dobrivojević Radmilović, Marina ; Škokić, Siniša ; Justić, Helena ; Barić, Anja ; Dugandžić, Aleksandra
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
12th FENS Forum of Neuroscience
Mjesto i datum
Virtualni kongres (Glasgow, Ujedinjeno Kraljevstvo), 11.07.2020. - 15.07.2020
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
guanylate cyclase-C ; middle cerebral artery occlusion ; MR imaging ; Ca2+ signaling
Sažetak
AIM: Recent research has shown that the activation of guanylate cyclase (GC)-A leads to a decrease of brain lesion size following middle cerebral artery occlusion (MCAO). Our aim was to investigate the potential role of uroguanylin (UGN)-dependent signalling pathways in the development of ischemic stroke. METHODS: Lesion volumes were measured in WT, GC-C and UGN-KO mice by MR imaging 1 and 7 days after MCAO. Intracellular Ca2+ concentrations were measured in slices of ipsilateral and contralateral hemispheres. RESULTS: GC-C-KO animals exhibited significantly smaller lesion volumes 1 day after MCAO than WT mice, with no difference 7 days later. This difference was not present between UGN-KO and WT littermates, although there was significant difference in UGN- dependent activation of Ca2+ signalling pathway in penumbral zone of ischemic lesion. CONCLUSION: The reduction in stroke volume present in GC-C-KO but not UGN-KO mice indicates that the activation of GC-C may cause an increase in ischemic lesion volumes, while GC-C-independent (Ca2+-dependent) signalling pathway may have protective effect. ACKNOWLEDGEMENTS Research was funded by the Scientific Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic- ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund). The work of doctoral student Anja Barić has been fully supported by the “Young researchers' career development project – training of doctoral students” and project BRADISCHEMIA (UIP-2017-05- 8082) of the Croatian Science Foundation funded by the European Union from the European Social Fund.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (ZCI Neuro) (Judaš, Miloš) ( CroRIS)
--UIP-2017-05-8082 - Uloga bradikinina u ishemiji mozga i mrežnice u mišjim modelima dijabetesa (BRADISCHEMIA) (Radmilović Dobrivojević, Marina) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Martina Ratko
(autor)
Aleksandra Sinđić
(autor)
Siniša Škokić
(autor)
Nikola Habek
(autor)
Anja Barić
(autor)