Pregled bibliografske jedinice broj: 1162743
Effects of Guanylate Cyclase-C on brain ischemic lesion formation
Effects of Guanylate Cyclase-C on brain ischemic lesion formation // FENS Regional Meeting
Beograd, Srbija, 2019. str. 246-246 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1162743 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Effects of Guanylate Cyclase-C on brain ischemic
lesion formation
Autori
Ratko, Martina ; Habek, Nikola ; Dobrivojević Radmilović, Marina ; Škokić, Siniša ; Justić, Helena ; Dugandžić, Aleksandra
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
FENS Regional Meeting
Mjesto i datum
Beograd, Srbija, 10.07.2019. - 13.07.2019
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Guanylate cyclase-C, uroguanylin, middle cerebral artery occlusion, ischemic stroke
Sažetak
Aims: Agonists of guanylate cyclase (GC) A but not GC-B are reducing the size of the brain lesion after an ischemic stroke. The aim of this study is to show if GC-C is involved in development of ischemic stroke. Methods: Middle cerebral artery occlusion (MCAO) was performed in wild type (WT) and GC-C knock-out (GC-C KO) mice. The volumes of brain lesions were determined 24h and 7 days after MCAO by MRI. In addition, patch clamp experiments and immunohistochemistry were performed. Results: Twenty-four hours after MCAO, GC-C KO animals have decreased lesion volumes compared to WT mice (WT:121±12 mm3, GC-C KO:79±14 mm3). Even though we showed a reduction in brain lesion 7 days after MCAO in WT mice, there was no change in GC-C KO mice and differences between mice were not present. Furthermore, GC-C agonist, uroguanylin, hyperpolarized cerebellar Purkinje neurons, (-7.2±1.4mV, n=5) while this effect was abolished in GC-C KO mice (1.6±1.7mV, n=4). Since most of the ischemic lesion of our mouse model is located in the cerebral cortex, it is not surprising that GC-C is present in neurons of cerebral cortex. Conclusion: Effects of GC-C activation on ischemic stroke are opposite to effects of GC-A agonists. GC-C KO mice develop smaller lesion volume 24h but not 7 days after an ischemic stroke compared to WT mice suggesting the possible benefit of specific GC-C inhibitors in treatment of ischemic stroke. Research was funded by the Scientific Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic-ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund) and Croatian Science Foundation project BRADISCHEMIA (UIP-2017-05-8082).
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0007 - Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi (ZCI Neuro) (Judaš, Miloš) ( CroRIS)
--UIP-2017-05-8082 - Uloga bradikinina u ishemiji mozga i mrežnice u mišjim modelima dijabetesa (BRADISCHEMIA) (Radmilović Dobrivojević, Marina) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb