Naslov
SELENIUM CONCENTRATIONS IN TYPE 2 DIABETES PATIENTS

Autori
Kozic, I. ; Perkovic Radojkovic, K. ; Bakula, M. ; Grubic, P. ; Rumenjak, V.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Poster Abstracts – IFCC WorldLab Istanbul 2014 / - : Clinical Chemistry and Laboratory Medicine, 2014, 678-678

Skup
22nd International Congress of Clinical Chemistry and Laboratory Medicine

Mjesto i datum
Istanbul, Turska, 22.06.2014. - 26.06.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
selenium ; type 2 diabetes ; blood sampling

Sažetak
BACKGROUND: Selenium is a trace element whose essentiality in mammals was discovered in 1957. Selenium defi-ciensies have been recognized as to be related to a number of pathologies such as cancer, cardiovascular diseases anddiabetes. Type 2 diabetes accounts for 90% of all diabetes and is characterized by peripheral insulin resistance with aninsulin secretory defect that varies in severity. Selenium is in human body mainly found in selenoproteins in the formof selenocysteine. Its primary functions are protection against oxidative stress (as a part of glutathion peroxidase) andimmune function. Oxidative stress has been suggested to play a main role in the pathogenesis of type 2 diabetes andits complications. Since we know selenium has an important role in protection against oxidative stress it is expectedthat its concentrations might be changed in type 2 diabetes. This study is to evaluate selenium levels in type 2 diabeticpatients compared to healthy controls. METHODS: Subjects We included 54 type 2 diabetes patients with various complications including hypertension, neph-ropaty, neuropathy and retinopathy. Patients with chronic kidney disease requiring dialysis were excluded. Fifty eightage- and sex-matched subjects were included for the control. Blood sampling. Venous peripheral blood samples werecollected after overnight fasting. Samples were centrifuged at 3500 rpm for 10 min and serum supernatants were sto-red on -30°C untill determination within 3 months. Selenium concentratios were determined using Inductively CoupledPlasma-Mass Spectrometry, ICP-MC.RESULTS: Mean values of selenium concentrations in type 2 diabetic patients (N=54) were 104.57±29, 17 μg/L. Meanvalue of selenium concentrations in control subjects (N=58) were 124.09±25, 15 μg/L. Using unpaired t-test (P<0, 05) itcan be seen there is significant difference in selenium concentrations between the two groups (P=0, 0002). CONCLUSIONS: From the results it can be seen that selenium concentrations in our group of type 2 diabetic patientswere significantly lower compared to healthy controls. Using that data we can assume that lower selenium concentra-tions subsuqently contribute to reduced protection against oxidative stress which facilitates onset of type 2 diabetesand its complications.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA