Pregled bibliografske jedinice broj: 1162174
Yeast RNA exosome activity is necessary for maintaining cell wall stability through proper protein glycosylation
Yeast RNA exosome activity is necessary for maintaining cell wall stability through proper protein glycosylation // Molecular Biology of the Cell, 32 (2021), 5; 363-375 doi:10.1091/mbc.e20-08-0544-t (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1162174 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Yeast RNA exosome activity is necessary for maintaining cell wall stability through proper protein glycosylation
Autori
Novačić, Ana ; Beauvais, Valentin ; Oskomić, Marina ; Štrbac, Lucija ; Le Dantec, Aurélia ; Rahmouni, A. Rachid ; Stuparević, Igor
Izvornik
Molecular Biology of the Cell (1059-1524) 32
(2021), 5;
363-375
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
RNA exosome ; Rrp6 ; yeast cell wall ; protein glycosylation
Sažetak
Nuclear RNA exosome is the main 3′→5′ RNA degradation and processing complex in eukaryotic cells and its dysregulation therefore impacts gene expression and viability. In this work we show that RNA exosome activity is necessary for maintaining cell wall stability in yeast Saccharomyces cerevisiae. While the essential RNA exosome catalytic subunit Dis3 provides exoribonuclease catalytic activity, the second catalytic subunit Rrp6 has a noncatalytic role in this process. RNA exosome cofactors Rrp47 and Air1/2 are also involved. RNA exosome mutants undergo osmoremedial cell lysis at high temperature or at physiological temperature upon treatment with cell wall stressors. Finally, we show that a defect in protein glycosylation is a major reason for cell wall instability of RNA exosome mutants. Genes encoding enzymes that act in the early steps of the protein glycosylation pathway are down-regulated at high temperature in cells lacking Rrp6 protein or Dis3 exoribonuclease activity and overexpression of the essential enzyme Psa1, that catalyzes synthesis of the mannosylation precursor, suppresses temperature sensitivity and aberrant morphology of these cells. Furthermore, this defect is connected to a temperature-dependent increase in accumulation of noncoding RNAs transcribed from loci of relevant glycosylation-related genes.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
UIP-2017-05-4411 - In silico i in vivo analiza transkriptoma stanične stijenke kvasca Saccharomyces cerevisiae i primjena dobivenih rezultata u konstrukciji novih biotehnoloških sojeva (SCCWTRANS) (Stuparevic, Igor, HRZZ - 2017-05) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE