Naslov
Oxidized proteins and selenium in patients with type 2 diabetes
(Oxidized proteins and selenium in patients with type 2 diabetes)

Autori
Kožić, I. ; Bakula, M. ; Grubic, P, Rumenjak V.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Skup sažetaka sa 14. EFLM simpozija u Dubrovniku, u: Biochemia Medica Vol. 24 (2014) / - , 2014, 60-60

Skup
14th EFLM Continuous Postgraduate Course in Clinical Chemistry and Laboratory Medicine

Mjesto i datum
Dubrovnik, Hrvatska, 25.10.2014. - 26.10.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oxidized proteins ; selenium ; diabetes mellitu

Sažetak
Background: Numerous studies suggest that the diabetic condition is associated with oxidative stress. Measurements of oxidized proteins are used as unspecific indicators of oxidative stress. The primary functions of selenium are defense against oxidative stress through selenium- dependent glutathione peroxidase and other selenoproteins.The purpose of this study is to evaluate selenium and oxidized protein levels in patients with type 2 diabetes compared to healthy controls. Materials and methods: Subjects. The study comprehended 54 patients with type 2 diabetes with various complications, including hypertension, nephropathy, neuropathy and retinopathy. Fifty eight age- and sex-matched subjects were included for the control. Selenium concentrations were determined using Inductively Coupled Plasma-Mass Spectrometry, ICP-MC (Perkin Elmer). Oxidized protein levels were determined using ELISA method with 2, 4- dinitrophenylhydrazine (DNP) (Biocell PC Test, BioCell Corporation, New Zealand). Results: Mean values of selenium in type 2 diabetic patients were 104.57±29, 17 µg/L. Mean values of selenium in control subjects were 124.09±25, 15 µg/L (P=0, 0002). Mean values of oxidized proteins in type 2 diabetic patients were 0.2585±0, 0511 nmol/mg. Mean values of oxidized proteins in control subjects were 0.1180±0.0623 nmol/mg (P=<0, 0001). Conclusion: From the results it can be seen that selenium concentrations in patients with type 2 diabetes were significantly lower compared to healthy controls. At the same time concentrations of oxidized proteins were significantly higher in patients with type 2 diabetes compared to healthy controls. Using that data we can assume that lower selenium concentrations subsequently contribute to reduced protection against oxidative stress which results in higher levels of oxidized proteins.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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