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Pregled bibliografske jedinice broj: 1160897

Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible


Tomljanović, Ivana; Petrović, Antonela; Ban, Jelena; Mladinic, Miranda
Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible // Biochemical and biophysical research communications, 587 (2022), 85-91 doi:10.1016/j.bbrc.2021.11.078 (međunarodna recenzija, kratko priopcenje, znanstveni)


CROSBI ID: 1160897 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible

Autori
Tomljanović, Ivana ; Petrović, Antonela ; Ban, Jelena ; Mladinic, Miranda

Izvornik
Biochemical and biophysical research communications (0006-291X) 587 (2022); 85-91

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
Monodelphis domestica ; Spinal cord ; Regeneration ; Proteomics ; Mass spectrometry ; Neurodegenerative diseases

Sažetak
One of the major challenges of modern neurobiology concerns the inability of the adult mammalian central nervous system (CNS) to regenerate and repair itself after injury. It is still unclear why the ability to regenerate CNS is lost during evolution and development and why it becomes very limited in adult mammals. A convenient model to study cellular and molecular basis of this loss is neonatal opossum (Monodelphis domestica). Opossums are marsupials that are born very immature with the unique possibility to successfully regenerate postnatal spinal cord after injury in the first two weeks of their life, after which this ability abbruptly stops. Using comparative proteomic approach we identified the proteins that are differentially distributed in opossum spinal tissue that can and cannot regenerate after injury, among which stand out the proteins related to neurodegenerative diseases (NDD), such as Huntington, Parkinson and Alzheimer's disease, previously detected by comparative transcriptomics on the analog tissue. The different distribution of the selected proteins detected by comparative proteomics was further confirmed by Western blot (WB), and the changes in the expression of related genes were analysed by quantitative reverse transcription PCR (qRT-PCR). Furthermore, we explored the cellular localization of the selected proteins using immunofluorescent microscopy. To our knowledge, this is the first report on proteins differentially present in developing, non-injured mammalian spinal cord tissue with different regenerative capacities. The results of this study indicate that the proteins known to have an important role in the pathophysiology of neurodegeneration in aged CNS, could also have an important phyisological role during CNS postnatal development and in neuroregeneration process.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija, Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-7060 - Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca (DefineREgenAgeMode) (Mladinić Pejatović, Miranda, HRZZ - 2016-06) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-prirod-18-290 1463 - Identifikacija i kvantifikacija neuralnih matičnih stanica novom metodom optimizirane homogenizacije (Ban, Jelena, NadSve - UNIRI projekti 2018) ( CroRIS)
Ostalo-CRP/CRO14-03 - Role of Activating Transcription Factor 3 and related molecules in the activation of spinal cord endogenous stem/progenitor cells (Mladinić Pejatović, Miranda, Ostalo - CRP - ICBEG Research Grants Programme 2014) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-258 6427 - Otkrivanje ključnih molekula koje kontroliraju stanične mehanizme neuroprotekcije i neuroregeneracije posredovane proteinima toplinskog stresa nakon in vitro ozljede leđne moždine oposuma (Mladinić Pejatović, Miranda, NadSve - UNIRI projekti 2018) ( CroRIS)
EK-EFRR-RC.2.2.06-0001 - Razvoj istraživačke infrastrukture na kampusu Sveučilišta u Rijeci (RISK) (Ožanić, Nevenka, EK - Operativni program Regionalna konkurentnost) ( CroRIS)

Ustanove:
Sveučilište u Rijeci - Odjel za biotehnologiju

Citiraj ovu publikaciju:

Tomljanović, Ivana; Petrović, Antonela; Ban, Jelena; Mladinic, Miranda
Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible // Biochemical and biophysical research communications, 587 (2022), 85-91 doi:10.1016/j.bbrc.2021.11.078 (međunarodna recenzija, kratko priopcenje, znanstveni)
Tomljanović, I., Petrović, A., Ban, J. & Mladinic, M. (2022) Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible. Biochemical and biophysical research communications, 587, 85-91 doi:10.1016/j.bbrc.2021.11.078.
@article{article, author = {Tomljanovi\'{c}, Ivana and Petrovi\'{c}, Antonela and Ban, Jelena and Mladinic, Miranda}, year = {2022}, pages = {85-91}, DOI = {10.1016/j.bbrc.2021.11.078}, keywords = {Monodelphis domestica, Spinal cord, Regeneration, Proteomics, Mass spectrometry, Neurodegenerative diseases}, journal = {Biochemical and biophysical research communications}, doi = {10.1016/j.bbrc.2021.11.078}, volume = {587}, issn = {0006-291X}, title = {Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible}, keyword = {Monodelphis domestica, Spinal cord, Regeneration, Proteomics, Mass spectrometry, Neurodegenerative diseases} }
@article{article, author = {Tomljanovi\'{c}, Ivana and Petrovi\'{c}, Antonela and Ban, Jelena and Mladinic, Miranda}, year = {2022}, pages = {85-91}, DOI = {10.1016/j.bbrc.2021.11.078}, keywords = {Monodelphis domestica, Spinal cord, Regeneration, Proteomics, Mass spectrometry, Neurodegenerative diseases}, journal = {Biochemical and biophysical research communications}, doi = {10.1016/j.bbrc.2021.11.078}, volume = {587}, issn = {0006-291X}, title = {Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible}, keyword = {Monodelphis domestica, Spinal cord, Regeneration, Proteomics, Mass spectrometry, Neurodegenerative diseases} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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