Naslov
CYTOTOXIC ACTIVITY OF THE COMMON JUNIPER ESSENTIAL OIL

Autori
Šimić, Anja ; Jažo, Zvonimir ; Parčina, Antonija ; Gusić, Klaudija ; Selimović, Samela ; Huseinović, Samira ; Bektić, Sanida, Čikeš Čulić, Vedrana ; Politeo, Olivera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
7th Croatian Meeting of Chemists and Chemical Engineers with international participation 5th Symposium Vladimir Prelog

Mjesto i datum
Veli Lošinj, Hrvatska, 05.10.2021. - 08.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
Juniperus communis, essential oil, GC-MS, cytotoxic activity

Sažetak
The common juniper (Juniperus communis L.) is a tall shrub or low tree that can grow up to 15 meters in height. This species grows in lowland to premountain areas, and has a natural distribution range across Asia, North America, Europe and North Africa. The fruit are small, round berries of dark blue color, 6-9 millimeters in diameter, which are harvested during autumn.[1] This paper investigates the cytotoxic activity of the common juniper essential oil extracted from its berries. The sampling of plant material was performed at the following locations in the territory of Bosnia and Herzegovina: Konjuh, Majevica, Srebrenica and Romanija. After drying, the extraction of essential oils from collected plant material was carried out in a 3- hour long process of hydrodistillation in a Clevenger apparatus. The content of essential oil in the berries was between 1.77 and 1.90%. The chemical composition of the essential oil was determined by an instrumental technique that combines gas chromatography and mass spectrometry (GC-MS). A total of 33 compounds were identified that make up 99.31-99.54% of the oil. The three most common compounds were determined as: α-Pinene (30.64–40.66%), Sabinene (21.82–26.02%) and β-Myrcene (6.14–11.74%). The cytotoxic effects of the common juniper essential oil was investigated on breast cancer cell lines (MDA-MB-231) and lung cancer (A549). Cell treatment was performed with essential oil that was dissolved in nutrient medium, with DMSO (<1%) used to improve oil solubility. Cell viability was determined relative to control cells by MTT reagent and by absorbance measurement at a wavelength of 570 nm. The estimated IC50 value in tests with breast cancer cells was 118.17–498 ug/mL after 24 h and 94.67–445 ug/mL after 48 h. In tests performed on lung cancer cells, the IC50 was 137.33–404 ug/mL after 24 h and 92–419 ug/mL after 48 h.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija

POVEZANOST RADA