Naslov
THE CYTOTOXIC EFFECT AND APOPTOSIS RATE OF BREAST CANCER STEM CELLS TREATED WITH THE NOVEL THIENO[2,3-B] PYRIDINE ANTICANCER COMPOUND
(Citotoksični učinak i apoptoza matičnih stanica karcinoma dojke tretiranih novosintetiziranim protutumorskim spojem tieno [2,3-b] piridinom)

Autori
Willmen, Louisa Pauline Sofie

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Medicinski fakultet

Mjesto
Split

Datum
15.07

Godina
2019

Stranica
60

Mentor
Čikeš Čulić, Vedrana

Ključne riječi
newly synthesized anticancer agent, triple-negative breast cancer, cancer stem cells
(novosintetizirani protutumorski spoj, trostruko negativni karcinom dojke, matične stanice karcinoma)

Sažetak
Objectives: The purpose of this study is to determine the effects of treating breast cancer stem cells with the newly synthesized thieno[2, 3- b]pyridine anticancer agent, by focusing on its cytotoxic and apoptotic effects on the investigated human cell line MDA-MB-231. Methods: The MDA-MB-231 triple-negative breast cancer cell line was treated with a newly developed thienopyridine anticancer compound (3- amino-N-(3-chloro-2-methylphenyl)-5- oxo-5, 6, 7, 8- tetrahydrothieno[2, 3-b]quinoline-2-carboxamide, 1) to determine its cytotoxic effect on triple negative breast cancer, the type of cell death it causes, and the cancer stem cell percentage after treatment. The 3-(4, 5-dimethylthiazolyl-2)-2, 5- diphenyltetrazolium bromide (MTT) assay was performed, to analyze the cellular metabolic activity and determine the cytotoxic effect. Flow cytometric analysis was used in combination with Annexin-V-FITC and propidium iodide staining to assess the type of cell death after 48h of treatment with compound 1 (2 µM). Furthermore, flow cytometry also provided the percentage of CD44+/CD24- cancer stem cells after treatment. Results: Compound 1 was cytotoxic for breast cancer cells in a dose- and time-dependent manner ; cell death occurs mainly by apoptosis. The percent of cancer stem cells decreased four times. Conclusion: Due to its cytotoxic effect on the percentage of triple negative breast cancer stem cells compound 1 can be a potential treatment for triple-negative breast cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA