Naslov
Tumor cytosol dipeptidyl peptidase III activity is increased with histological aggressiveness of ovarian primary carcinomas

Autori
Šimaga, Šumski ; Babić, Damir ; Osmak, Maja ; Šprem, Marina ; Abramić, Marija

Izvornik
Gynecologic Oncology (0090-8258) 91 (2003); 194 - 200

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
dipeptidyl peptidase III; human ovary; ovarian cancer
(dipeptidyl pepitdase III; human ovary; ovarian cancer)

Sažetak
Objective. Proteolytic enzymes have been implicated in the progression of various human malignancies, including ovarian cancer. The enhanced expression of dipeptidyl peptidase III (DPP III) was found in endometrial carcinomas of various histological types and grade. The aim of this study was to assess activity of DPP III in ovarian tissue specimens and to correlate it to clinico pathological data. Methods. DPP III hydrolytic activity towards Arg-Arg-2-naphthylamide was determined in 108 ovarian tissue cytosol specimens of seventy nine patients. The data obtained for 41 ovarian primary carcinoma specimens were stratified according to clinical stage, histological grade and type, and the age of the patients. Results. Median DPP III activity expressed as mU/mg protein was 6.0 in normal ovarian tissues (n=29), 6.5 in benign ovarian tumors (n=19), 19.5 in primary ovarian carcinomas (n=41), 12.5 in non-epithelial primary ovarian tumors (n=7) and 22.1 in metastatic ovarian malignancies (n=12). A significant rise in median DPP III specific activity was observed in malignant ovarian tumors (of epithelial, non-epithelial and metastatic origin), but not in benign ovarian tumors, compared to the activity in normal tissue. A significant difference of DPP III expression was found between the group of normal tissues and tumors of clinical stage I and II, of grade 2 and 3, of serous and mucinous histologic type. Conclusions. DPP III activity of benign ovarian tumors equaled that in normal ovarian tissue. In malignant neoplasms of the ovary it increased with growing histologic grade.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA