Naslov
Elucidation of Enzymes Suitable for Lignin Degradation
(Elucidation of Enzymes Suitable for Lignin Degradation (Istraživanje enzima prikladnih za razgradnju lignina))

Autori
Ramljak, Sanja

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prehrambeno-tehnološki fakultet Osijek

Mjesto
Osijek

Datum
27.11

Godina
2020

Stranica
53

Mentor
Velić, Natalija ; Ludwig, Roland

Ključne riječi
kraft lignin, laccase, Trametes versicolor, phanerochaete chrysosporium

Sažetak
Enzymatic degradation of kraft lignin was investigated using purified fungal enzymes known to degrade lignin structures. The activity of laccase, the most frequent enzyme associated with ligninolysis and the activity of auxiliary redox enzymes such as glucose dehydrogenase (GDH), cellobiose dehydrogenase (CDH) and lytic polysaccharide monooxygenase (LPMO) were tested. The effectiveness of these purified enzymes was compared with crude extracts obtained from fungal cultivations of the ligninolytic model fungi: Trametes versicolor and Phanerochaete chrysosporium. Laccase was proven to be the most efficient enzyme for lignin degradation. Auxiliary enzymes cooperate with other enzymes in order to enhance depolymerisation of lignocellulose. With observed high laccase activity, Trametes versicolor is found to be efficient in lignin degradation.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA