Pregled bibliografske jedinice broj: 1156625
Determination of N-Acetyl-l-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
Determination of N-Acetyl-l-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands // Molecules, 26 (2021), 22; 6826, 9 doi:10.3390/molecules26226826 (međunarodna recenzija, članak, znanstveni)
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Naslov
Determination of N-Acetyl-l-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands
Autori
Kukoc-Modun, Lea ; Kraljević, Tomislav, Tsikas, Dimitrios ; Radić, Njegomir ; Modun, Darko
Izvornik
Molecules (1420-3049) 26
(2021), 22;
6826, 9
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
N-acetyl-L-cysteine ethyl ester ; neocuproine ; bicinchoninic acid ; bathocuproine disulfonic acid ; flow injection analysis ; spectrophotometric determination
Sažetak
A new flow injection spectrophotometric method for the determination of N-acetyl-l-cysteine ethyl ester (NACET) was developed and validated. The method is based on the reduction of Cu(II)-ligand complexes to chromophoric Cu(I)-ligand complexes with the analyte. The studied ligands were neocuproine (NCN), bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The absorbance of the Cu(I)-ligand complex was measured at 458, 562 and 483 nm for the reactions of NACET with NCN, BCA and BCS, respectively. The method was validated in terms of linear dynamic range, limit of detection and quantitation, accuracy, selectivity, and precision. Experimental conditions were optimized by a univariate method, yielding linear calibration curves in a concentration range from 2.0 × 10−6 mol L−1 to 2.0 × 10−4 mol L−1 using NCN ; 2.0 × 10−6 mol L−1 to 1.0 × 10−4 mol L−1 using BCA and 6.0 × 10−7 mol L−1 to 1.2 × 10−4 mol L−1 using BCS. The achieved analytical frequency was 90 h−1 for all three ligands. The method was successfully employed for NACET determination in pharmaceutical preparations, indicating that this FIA method fulfilled all the essential demands for the determination of NACET in quality control laboratories, as it combined low instrument and reagent costs with a high sampling rate.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Farmacija
POVEZANOST RADA
Ustanove:
Kemijsko-tehnološki fakultet, Split,
Medicinski fakultet, Split
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE