Naslov
Application of MALDI-TOF-MSI method on 3D cell models

Autori
Filipović, Nikolina ; Marković, Berislav ; Debeljak Željko ; Mišković Špoljarić, Katarina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts 27th Croatian Meeting of Chemists and Chemical Engineers / - , 2021, 188-188

Skup
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI)

Mjesto i datum
Veli Lošinj, Hrvatska, 05.10.2021. - 08.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
spectroscopy, 3D cell model, MALDI, TOF-MSI, organometallic compounds

Sažetak
Mass spectrometry with imaging (MSI) is an extremely powerful method that, in addition to being a research analytical technique, can also be used for targeted detection of significant molecular species. Unlike classical visualization methods, by using MSI technique, it is possible to identify hundreds of analytes simultaneously. The addition of TOF (Time of Flight) allows ion flight time to differentiate according to the value of ion mass/charge. Consequently - ions with lower m/z values travel faster. Characteristics such as the ability to scan a sample up to 20 times make this technique excellent for monitoring and reviewing the spatial distribution of different molecular species in many cell and tissue structures. Cells grown as 3D cultures are very attractive model for study in biological researches. The reason for this is the greater compatibility with in vivo conditions, since cells are cultured in the form of the spheroids whose microenvironment is similar to that in living tissues.1-2 The above method with all its advantages will be used to detect the effect of organometallic compounds on 3D models of ¬¬¬Caco- 2 and Hep-G2 cell lines.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA