Naslov
What hematological parameters in dogs are affected when the blood is lipemic?

Autori
Đurić, Vlasta ; Kučer, Nada ; Brkljačić, Mirna ; Ivšić Škoda, Dora ; Kiš, Ivana ; Dodig, Tea ; Ećimović, Luka ; Subašić, Nejra ; Beer Ljubić, Blanka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 9th International Congress Veterinary Science and Profession / Brkljača Bottegaro, Nika ; Lukač, Maja ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2021, 87-87

ISBN
978-953-8006-36-4

Skup
9th International Congress: Veterinary Science and Profession

Mjesto i datum
Zagreb, Hrvatska, 09.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
canine blood ; lipemia ; hematological parameters

Sažetak
Interferences are an important source of results variability in laboratory diagnostics. Lipemia is considered second most often interference in hematology in veterinary laboratory medicine, after hemolysis. Aim of this study is to determine what complete blood count (CBC) parameters in dogs are affected by lipemia. The study was conducted on 10 dogs. Blood samples were collected from cephalic vein into EDTA tubes. Blood samples were neither lipemic nor hemolytic. The complete blood count was performed on Horiba Scil vet ABC Plus+ hematological analyzer (Scil, Germany). SMOFLipid 20% solution was used for sample preparation of lipemia interference. Diluted lipid solutions were prepared according to lipemic index (Abbott Architect biochemical analyzer: 0, 1+, 2+, 3+, 4+) and final lipid concentrations were 0.3, 0.9, 1.4, 2.0 and 5.0 g/L. Lipid solution was added to whole blood sample aliquot after performing initial hematological analysis. Measurements were done in duplicates. Results indicate that lipemia statistically significantly increased hemoglobin (HB), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in dog samples (p<0.05). Red blood cell count (RBC), hematocrit (HTC), mean corpuscular volume (MCV) and red cell distribution width (RDW) were not changed significantly with increase of lipemia and differences were less than 5%. White blood cell count (WBC) and platelet count (PLT) increased with lipemia, with differences from 11% to 30% and 8% to 24% respectively (p>0.05 both). Mean platelet volume (MPV) decreased from 2% to 8% with increasing of lipid concentration. In conclusion, RBC, HTC and MCV can be measured reliably in lipemic samples. Due to turbidity of a sample HB, MCH and MCHC should not be assayed in lipemic samples. The determination of WBC, PLT and MPV in lipemic samples results in approximate values.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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