Pregled bibliografske jedinice broj: 1154815
Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin
Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo- transferrin, 2020. (ostalo).
CROSBI ID: 1154815 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Protocol for ultra performance liquid
chromatography-mass spectrometry N-glycan analysis
of the native and desialylated human apo-
transferrin
Autori
Štambuk, Tamara ; Cvetko, Ana ; Weitner, Tin
Vrsta, podvrsta
Ostale vrste radova, ostalo
Godina
2020
Ključne riječi
transferrin ; N-glycan analysis ; UPLC ; mass spectrometry
Sažetak
This protocol describes the method used for N- glycan analysis of the native and desialylated human apo-transferrin. The protein N-glycans were released with the addition of 1.2 U of PNGase F and overnight incubation at 37°C. The released N-glycans were labeled with 2-AB and purified using hydrophilic interaction liquid chromatography solid-phase extraction (HILIC-SPE). Fluorescently labeled N-glycans were separated by UPLC using Waters BEH Glycan chromatography column. All glycan structures were annotated with MS/MS analysis using an ESI-QTOF-MS system
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Farmacija
POVEZANOST RADA
Projekti:
UIP-2017-05-9537 - Glikozilacija serumskog transferina kao faktor u mehanizmu prijenosa željeza (GlyMech) (Weitner, Tin, HRZZ - 2017-05) ( CroRIS)
