Pregled bibliografske jedinice broj: 1154651
Strawberry tree (Arbutus unedo L.) honey extract ameliorates genotoxic effects of irinotecan but leads to cell cycle delays in human peripheral lymphocytes in vitro
Strawberry tree (Arbutus unedo L.) honey extract ameliorates genotoxic effects of irinotecan but leads to cell cycle delays in human peripheral lymphocytes in vitro // Abstracts of the 6th Croatian Congress of Toxicology with International Participation CROTOX 2021 ; u: Arhiv za higijenu rada i toksikologiju 72 (2021) (S1) / Lyons, Daniel Mark (ur.).
Zagreb: Institut za medicinska istraživanja i medicinu rada, 2021. str. 57-57 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 1154651 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Strawberry tree (Arbutus unedo L.) honey extract
ameliorates genotoxic effects of irinotecan but
leads to cell cycle delays in human peripheral
lymphocytes in vitro
Autori
Jurič, Andreja ; Huđek Turković, Ana ; Brčić Karačonji, Irena ; Lušić, Dražen ; Durgo, Ksenija ; Kopjar, Nevenka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Abstracts of the 6th Croatian Congress of Toxicology with International Participation CROTOX 2021 ; u: Arhiv za higijenu rada i toksikologiju 72 (2021) (S1)
/ Lyons, Daniel Mark - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2021, 57-57
Skup
6th Croatian congress of toxicology with international participation (CROTOX 2021)
Mjesto i datum
Rabac, Hrvatska, 03.10.2021. - 06.10.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
chromosomal aberrations, micronucleus assay, cyto-/genoprotective effects, lymphocytes
Sažetak
This study assessed genotoxic profile of strawberry tree honey extract (STHE) (i.e. extracted phenolic fraction of STH) and its potential protective effects against cytogenetic damage induced by the anticancer drug irinotecan (IRI) in human peripheral lymphocytes in vitro. Isolated lymphocytes were exposed for 2 h to three different concentrations of STHE (corresponding to average daily portion of 50 g of honey, as well as five- and ten- fold higher concentration) alone and in combination with IRI. Concentration of IRI corresponded to its therapeutic dose of 350 mg/m2. The outcomes of the treatments were evaluated using the analysis of chromosomal aberrations and the cytokinesis-block micronucleus (CBMN) cytome assay. Although STHE increased incidence of chromosomal aberrations compared to negative control at all tested concentrations, when administered with IRI, it showed significant protective effects. Single STHE did not produce significant changes in number of MN and nuclear buds (NB) compared to negative control. When given in combination with tested anticancer drug, STHE effectively diminished incidence of both MN and NB compared to single IRI. The most prominent effect of STHE observed both after single and combined treatments was its cell- cycle delaying activity, which resulted with significantly lowered values of the replication index. Observed cytostatic effect could be associated with chemical composition of the tested extract, whose constituents exhibited both prooxidative and antioxidative properties. Convincing results obtained in vitro speak in favour of future investigations of STH, and further development of STH-based nutraceuticals, potentially useful even in cancer patients.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita
POVEZANOST RADA
Projekti:
Zaklada HAZU--10-1021-274-2020 - Biološki učinci meda obične planike (Arbutus unedo L.) na tumorske i zdrave ljudske stanice (Brčić Karačonji, Irena, Zaklada HAZU ) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Medicinski fakultet, Rijeka,
Fakultet zdravstvenih studija u Rijeci
Profili:
Ksenija Durgo
(autor)
Ana Huđek Turković
(autor)
Nevenka Kopjar
(autor)
Dražen Lušić
(autor)
Irena Brčić Karačonji
(autor)
Andreja Jurič
(autor)