Naslov
Co-infection with Dirofilaria repens and Dirofilaria immitis in a dog - a case report

Autori
Lovrić, Lea ; Mihoković Buhin, Ivana ; Mojčec Perko, Vesna ; Živičnjak Tatjana ; Štritof, Zrinka ; Beck, Relja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th International Congress “Veterinary Science and Profession” Book of Abstracts / Brkljača Bottegaro, Nika ; Lukač, Maja ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2021, 84-84

ISBN
978-953-8006-36-4

Skup
9th International Congress: Veterinary Science and Profession

Mjesto i datum
Zagreb, Hrvatska, 09.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dirofilaria, microfilariae, PCR, dog

Sažetak
Canine dirofilarioses are mosquito-borne zoonoses with a continuous expansion in Europe. While Dirofilaria immitis poses a great risk to animal health causing heartworm disease with a potentially fatal outcome, Dirofilaria repens usually causes non-pathogenic subcutaneous dirofilariosis. Over the past decade, an increasing number of cases of D. immitis and D. repens infections in dogs and humans have been reported. The following case describes accidental finding of D. immitis and D. repens co-infection in a dog. In 2021, a 9-year-old male dog from the city of Sisak was autopsied at the Faculty of Veterinary Medicine University of Zagreb. Three adult nematodes were found in the right ventricle of the heart. One nematode and blood clot from the right heart were collected for further examination. Modified Knott’s test was performed from mechanically lysed blood clot and revealed 11 050 microfilariae/ml. The same sample was screened for D. immitis circulating antigen using FASTest® HW Antigen (MEGACOR) and yielded negative. After DNA extraction from nematode, blood clot and lysed blood clot using NucleoSpin Tissue (Macherey-Nagel, Düren, Germany), PCR amplification of a cytochrome oxidase subunit 1 (COI) gene fragment of D. immitis and D. repens showed positivity for both species in blood clot samples, while nematode was positive for D. immitis. Previous studies suggest that D. immitis and D. repens has expanded in Europe in the last decade due to increasing movement of dogs and global climate changes. In Croatia, D. repens was found to be widespread throughout country, while D. immitis was found mainly in the coastal area of Croatia, but also in the continental parts including the city of Sisak. Knott’s test is considered the gold standard for detection of microfilariae, but detecting mixed infections can be challenging because of the morphological similarities between microfilariae of both nematodes. Therefore, molecular diagnostic tests are recommended to use.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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