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Pregled bibliografske jedinice broj: 1154522

Extracellular laccase activity of Cryphonectria hypovirus 1 – infected chestnut blight fungus


Nuskern, Lucija; Tkalec, Mirta; Srezović Bijelić, Bruno; Ježić, Marin; Ćurković-Perica, Mirna
Extracellular laccase activity of Cryphonectria hypovirus 1 – infected chestnut blight fungus // III International Green Biotechnology Congress
Sarajevo, Bosna i Hercegovina, 2021. str. 26-26 (predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1154522 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Extracellular laccase activity of Cryphonectria hypovirus 1 – infected chestnut blight fungus

Autori
Nuskern, Lucija ; Tkalec, Mirta ; Srezović Bijelić, Bruno ; Ježić, Marin ; Ćurković-Perica, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
III International Green Biotechnology Congress

Mjesto i datum
Sarajevo, Bosna i Hercegovina, 30.09.2021. - 02.10.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Biocontrol, enzyme, fungus-virus interaction

Sažetak
Cryphonectria parasitica is an invasive phytopathogenic fungus, the causal agent of chestnut blight - a devastating disease of chestnut trees in Europe and North America. The disease can be controlled by a mycovirus Cryphonectria hypovirus 1 (CHV1), which is either present in fungal populations naturally or introduced artificially. CHV1 reduces the virulence and fertility of C. parasitica, a phenomenon called hypovirulence which positively affects recovery of the infected trees. Laccase, a polyphenol oxidase, secreted by the chestnut blight fungus C. parasitica, is thought to be involved in pathogenesis towards chestnut due to its ability to degrade lignin. Infection with CHV1 has been shown to reduce laccase activity of the infected fungus, but the research has been done only on limited number of fungal isolates and virus strains. Because the role in pathogenesis could have important implications in selection of the best virus strains for human aided biocontrol of chestnut blight, our aim was to determine the effect of CHV1 strains belonging to different virus subtypes on laccase activity in different C. parasitica isolates. Four CHV1 strains (belonging to three different subtypes) were transferred to three fungal isolates by hyphal anastomosis. The cultures were subsequently grown on potato dextrose broth. Extracellular laccase activity of virus-free (control) and virus-infected C. parasitica isolates was assayed with 2, 6- dimethoxyphenol as a substrate. In two C. parasitica strains CHV1 infection expectedly reduced extracellular laccase activity, while in one C. parasitica strain, regardless of the virus strain utilized, laccase activity was surprisingly elevated. These results indicate that extracellular laccase activity of chestnut blight fungus is affected by factors beyond CHV1 infection, demonstrating that some C. parasitica isolates might exhibit unusual and unexpected response to virus infection.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IZHRZ0_180651 - Dynamics of virus infection in mycovirus-mediated biological control of a fungal pathogen (DynaMyco) (Ježić, Marin, HRZZ - Croatian-Swiss Research Programme 2017 - 2023) ( CroRIS)
HRZZ-IP-2013-11-5381 - Raznolikost i interakcije kestena, patogene gljive koja izaziva rak kestena i virusa koji inficira gljivu: učinak na oporavak kestena (Chestnut) (Ćurković Perica, Mirna, HRZZ - 2013-11) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

drive.google.com

Citiraj ovu publikaciju:

Nuskern, Lucija; Tkalec, Mirta; Srezović Bijelić, Bruno; Ježić, Marin; Ćurković-Perica, Mirna
Extracellular laccase activity of Cryphonectria hypovirus 1 – infected chestnut blight fungus // III International Green Biotechnology Congress
Sarajevo, Bosna i Hercegovina, 2021. str. 26-26 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Nuskern, L., Tkalec, M., Srezović Bijelić, B., Ježić, M. & Ćurković-Perica, M. (2021) Extracellular laccase activity of Cryphonectria hypovirus 1 – infected chestnut blight fungus. U: III International Green Biotechnology Congress.
@article{article, author = {Nuskern, Lucija and Tkalec, Mirta and Srezovi\'{c} Bijeli\'{c}, Bruno and Je\v{z}i\'{c}, Marin and \'{C}urkovi\'{c}-Perica, Mirna}, year = {2021}, pages = {26-26}, keywords = {Biocontrol, enzyme, fungus-virus interaction}, title = {Extracellular laccase activity of Cryphonectria hypovirus 1 – infected chestnut blight fungus}, keyword = {Biocontrol, enzyme, fungus-virus interaction}, publisherplace = {Sarajevo, Bosna i Hercegovina} }
@article{article, author = {Nuskern, Lucija and Tkalec, Mirta and Srezovi\'{c} Bijeli\'{c}, Bruno and Je\v{z}i\'{c}, Marin and \'{C}urkovi\'{c}-Perica, Mirna}, year = {2021}, pages = {26-26}, keywords = {Biocontrol, enzyme, fungus-virus interaction}, title = {Extracellular laccase activity of Cryphonectria hypovirus 1 – infected chestnut blight fungus}, keyword = {Biocontrol, enzyme, fungus-virus interaction}, publisherplace = {Sarajevo, Bosna i Hercegovina} }




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