Naslov
Metoda izdvajanja dnk na zavodu za mobilnu protetiku Stomatološkog fakulteta u Zagrebu
(The establishment of the dna isolation method at the department of removable prosthodontics, University of Zagreb, School of dental medicine)

Autori
Zlendić, Marko ; Vrbanović, Ema ; Gall Trošelj, Koraljka ; Tomljanović, Marko ; Alajbeg Z., Iva

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
7. Međunarodni kongres Stomatološkog fakulteta Sveučilišta u Zagrebu

Mjesto i datum
Rovinj, Hrvatska, 21.05.2021. - 22.05.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
polimorfizmi gena ; deoksiribonukleinska kiselina ; obrisak sluznice obraza ; temporomandibularni poremećaji ; lančana reakcija polimerazom
(polymorphisms ; deoxyribonucleic acid ; buccal swab ; temporomandibular disorders ; polymerase chain reaction)

Sažetak
Introduction: To establish a rapid and simple DNA isolation method from buccal swabs of patients with temporomandibular disorders (TMD) for the future analyses of specific polymorphisms in selected genes. Materials and methods: Buccal swabs were obtained from 40 subjects (22 TMD and 18 control patients) who agreed to participate in the study. Sample processing and DNA isolation were performed using a DNA isolation kit ChargeSwitch® PCR Clean-Up Kit(Carlsbad, United States, Life Technologies) by the research group at the Department of Removable Prosthodontics. The procedure of DNA isolation and purification relies on the use of magnetic beads. DNA quality was checked by 1% gel electrophoresis and polymerase chain reaction (PCR) amplification of exon 11 of the RET protooncogen, at the Rudjer Boskovic Institute. Results: A satisfying amount of DNA was obtained from all 40 buccal mucosal swabs. Samples will be further used for genotypization of polymorphism, which may be associated with the etiology of temporomandibular disorders, using the TaqMan™ SNP Genotyping Assay chemistry (Carlsbad, United States, Life Technologies). Conclusion: The presented method has proven to be a good option, which will be used in the future research. Support: Croatian Science Foundation Project IP- 2019-04-6211 (PI: Iva Alajbeg) and “Young Researchers’ Career Development Project - Training of Doctoral Students” (DOK-2020-01) (student: Marko Zlendić) Key words: polymorphisms, deoxyribonucleic acid, buccal swab, temporomandibular disorders, polymerase chain reaction

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA