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Pregled bibliografske jedinice broj: 1153396

Mass spectrometry-based methods for immunoglobulin G N-glycosylation analysis

Habazin, Siniša; Štambuk, Jerko; Šimunović, Jelena; Keser, Toma; Razdorov, Genadij; Novokmet, Mislav
Mass spectrometry-based methods for immunoglobulin G N-glycosylation analysis // Antibody glycosylation / Pezer, Marija (ur.).
Cham: Springer, 2021. str. 73-135 doi:10.1007/978-3-030-76912-3_3

CROSBI ID: 1153396 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Mass spectrometry-based methods for immunoglobulin G N-glycosylation analysis

Autori
Habazin, Siniša ; Štambuk, Jerko ; Šimunović, Jelena ; Keser, Toma ; Razdorov, Genadij ; Novokmet, Mislav

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, stručni

Knjiga
Antibody glycosylation

Urednik/ci
Pezer, Marija

Izdavač
Springer

Grad
Cham

Godina
2021

Raspon stranica
73-135

ISSN
1664-431X

Ključne riječi
mass spectrometry ; liquid chromatography ; solid-phase extraction ; ion fragmentation ; glycomics ; glycoproteomics ; biomarker discovery ; antibodies ; immunoglobulins

Sažetak
Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis. Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization. This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N- glycosylation analysis on several complexity levels. Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks. Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography–mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Projekti:
EK-KF-KK.01.1.1.01.0010 - Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju (ZCIPersonHealth) (Polašek, Ozren; Secenji, Aleksandar, EK ) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
GENOS d.o.o.

Profili:

Avatar Url Siniša Habazin (autor)

Avatar Url JERKO ŠTAMBUK (autor)

Avatar Url Mislav Novokmet (autor)

Avatar Url Toma Keser (autor)

Avatar Url Jelena Šimunović (autor)

Avatar Url Genadij Razdorov (autor)

Poveznice na cjeloviti tekst rada:

doi link.springer.com link.springer.com

Citiraj ovu publikaciju:

Habazin, Siniša; Štambuk, Jerko; Šimunović, Jelena; Keser, Toma; Razdorov, Genadij; Novokmet, Mislav
Mass spectrometry-based methods for immunoglobulin G N-glycosylation analysis // Antibody glycosylation / Pezer, Marija (ur.).
Cham: Springer, 2021. str. 73-135 doi:10.1007/978-3-030-76912-3_3
Habazin, S., Štambuk, J., Šimunović, J., Keser, T., Razdorov, G. & Novokmet, M. (2021) Mass spectrometry-based methods for immunoglobulin G N-glycosylation analysis. U: Pezer, M. (ur.) Antibody glycosylation. Cham, Springer, str. 73-135 doi:10.1007/978-3-030-76912-3_3.
@inbook{inbook, author = {Habazin, Sini\v{s}a and \v{S}tambuk, Jerko and \v{S}imunovi\'{c}, Jelena and Keser, Toma and Razdorov, Genadij and Novokmet, Mislav}, editor = {Pezer, M.}, year = {2021}, pages = {73-135}, DOI = {10.1007/978-3-030-76912-3\_3}, keywords = {mass spectrometry, liquid chromatography, solid-phase extraction, ion fragmentation, glycomics, glycoproteomics, biomarker discovery, antibodies, immunoglobulins}, doi = {10.1007/978-3-030-76912-3\_3}, issn = {1664-431X}, title = {Mass spectrometry-based methods for immunoglobulin G N-glycosylation analysis}, keyword = {mass spectrometry, liquid chromatography, solid-phase extraction, ion fragmentation, glycomics, glycoproteomics, biomarker discovery, antibodies, immunoglobulins}, publisher = {Springer}, publisherplace = {Cham} }
@inbook{inbook, author = {Habazin, Sini\v{s}a and \v{S}tambuk, Jerko and \v{S}imunovi\'{c}, Jelena and Keser, Toma and Razdorov, Genadij and Novokmet, Mislav}, editor = {Pezer, M.}, year = {2021}, pages = {73-135}, DOI = {10.1007/978-3-030-76912-3\_3}, keywords = {mass spectrometry, liquid chromatography, solid-phase extraction, ion fragmentation, glycomics, glycoproteomics, biomarker discovery, antibodies, immunoglobulins}, doi = {10.1007/978-3-030-76912-3\_3}, issn = {1664-431X}, title = {Mass spectrometry-based methods for immunoglobulin G N-glycosylation analysis}, keyword = {mass spectrometry, liquid chromatography, solid-phase extraction, ion fragmentation, glycomics, glycoproteomics, biomarker discovery, antibodies, immunoglobulins}, publisher = {Springer}, publisherplace = {Cham} }

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