Pregled bibliografske jedinice broj: 1151904
Rapid Structure Determination of Bioactive 4''- tetrahydrofurfuryl Macrozone Reaction Mixture Components by LC/SPE/Cryo NMR and MS
Rapid Structure Determination of Bioactive 4''- tetrahydrofurfuryl Macrozone Reaction Mixture Components by LC/SPE/Cryo NMR and MS // Molecules, 26 (2021), 6316; 26206316, 11 doi:10.3390/molecules26206316 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1151904 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Rapid Structure Determination of Bioactive 4''-
tetrahydrofurfuryl Macrozone Reaction Mixture
Components by LC/SPE/Cryo NMR and MS
Autori
Habinovec, Iva ; Mikulandra, Ivana ; Sekula, Lucia Ema ; Gašperov, Jana ; Kazazić, Saša ; Novak, Predrag
Izvornik
Molecules (1420-3049) 26
(2021), 6316;
26206316, 11
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
azithromycin conjugates ; macrozones ; HPLC-SPE/cryo NMR ; MS ; structure determination
Sažetak
LC-SPE/cryo NMR and MS methodologies have been developed and employed for a rapid structure determination of 4″-tetrahydrofurfuryl macrozone reaction mixture components. Macrozones, novel conjugates of azithromycin, and thiosemicarbazones have shown very good in vitro antibacterial activities against susceptible and some resistant bacterial strains and are promising agents for further development. The post-column multiple trapping of the chromatographically separated reaction mixture components on the SPE cartridges increased the sensitivity and together with cryogenically cooled NMR probe made it possible to identify and structurally characterize main 4″- tetrahydrofurfuryl macrozone reaction mixture compounds including those present at very low concentration level. This approach has several advantages over a classical off-line procedure, efficiency and low solvent consumption being the two most important ones. All identified components were process-related. It has been demonstrated that two different kinds of compounds with respect to structure were identified, i.e., macrolide- related and thiosemicarbazone-related ones. This methodology can serve as a platform for reliable and effective macrolides reaction components structure profiling, serving as both isolation and identification tools.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
HRZZ-IP-2018-01-8098 - Makrozoni, novi konjugati makrolidnih antibiotika: Dizajn, priprava i interakcije (Makrozoni/Macrozones) (Novak, Predrag, HRZZ - 2018-01) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE