Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques

Elez Garofulić, Ivona; Malin, Valentina; Repajić, Maja; Zorić, Zoran; Pedisić, Sandra; Sterniša, Meta; Smole Možina, Sonja; Dragović-Uzelac, Verica

doi:10.3390/molecules26206153

Pregled bibliografske jedinice broj: 1150736

Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques

Elez Garofulić, Ivona; Malin, Valentina; Repajić, Maja; Zorić, Zoran; Pedisić, Sandra; Sterniša, Meta; Smole Možina, Sonja; Dragović-Uzelac, Verica

Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques // Molecules, 26 (2021), 20; 6153, 16 doi:10.3390/molecules26206153 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1150736 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Phenolic profile, antioxidant capacity and antimicrobial activity of nettle leaves extracts obtained by advanced extraction techniques

Autori
Elez Garofulić, Ivona ; Malin, Valentina ; Repajić, Maja ; Zorić, Zoran ; Pedisić, Sandra ; Sterniša, Meta ; Smole Možina, Sonja ; Dragović-Uzelac, Verica

Izvornik
Molecules (1420-3049) 26 (2021), 20; 6153, 16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
nettle leaves ; microwave-assisted extraction ; pressurized liquid extraction ; advanced extraction techniques ; plant polyphenols ; antioxidant capacity ; ORAC ; UPLC MS2 ; antimicrobial activity

Sažetak
Nettle is a widely known plant whose high biological activity and beneficial medicinal effects are attributed to various bioactive compounds, among which polyphenols play an important role. In order to isolate polyphenols and preserve their properties, advanced extraction techniques have been applied to overcome the drawbacks of conventional ones. Therefore, microwave-assisted extraction (MAE) has been optimized for the isolation of nettle leaves polyphenols and it was compared to pressurized liquid extraction (PLE) and conventional heat-reflux extraction (CE). The obtained extracts were analyzed for their individual phenolic profile by UPLC MS2 and for their antioxidant capacity by ORAC assay. MAE proved to be the more specific technique for the isolation of individual phenolic compounds, while PLE produced extracts with higher amount of total phenols and higher antioxidant capacity. Both techniques were more effective compared to CE. PLE nettle extract showed antimicrobial activity against bacteria, especially against Gram-negative Pseudomonas fragi ATCC 4973 and Campylobacter jejuni NCTC 11168 strains. This suggests that PLE is suitable for obtaining a nettle extract with antioxidant and antimicrobial potential, which as such has great potential for use as a value-added ingredient in the food and pharmaceutical industry.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA

Projekti:
IP-2018-01-4924 - Izolacija i enkapsulacija bioaktivnih molekula samonikle i kultivirane koprive i komorača i učinci na fiziologiju organizma (PlantBioPower) (Dragović-Uzelac, Verica, HRZZ - 2018-01) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Verica Dragović-Uzelac (autor)