Naslov
Differently coated silver nanoparticles cause oxidative stress and induce cellular damage in tobacco (Nicotiana tabacum) seedlings

Autori
Biba, Renata ; Cvjetko, Petra ; Tkalec, Mirta ; Košpić, Karla ; Peharec Štefanić, Petra ; Lyons, Daniel Mark ; Balen, Biljana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 6th Croatian Congress of Toxicology with International Participation CROTOX 2021, Arhiv za higijenu rada i toksikologiju 2021 ; 72, Suppl. 1 / Lyons, Daniel M. ; Brčić Karačonji, Irena ; Kopjar, Nevenka ; Herman, Makso - , 2021, 33-33

Skup
6th Croatian congress of toxicology with international participation (CROTOX 2021)

Mjesto i datum
Rabac, Hrvatska, 03.10.2021. - 06.10.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
AgNP-CTAB ; AgNP-PVP ; cell viability ; glutathione ; reactive oxygen species

Sažetak
The novel characteristics of silver nanoparticles (AgNPs) have broadened their application in various industrial sectors, increasing the chance of their release into the environment and posing a risk for human health. Although AgNPs tend to oxidize and aggregate in aqueous media, they may be stabilized through surface coatings that can change their physico-chemical properties and affect their toxicity. This study compares effects of two differently coated AgNPs [polyvinylpyrrolidone (AgNP-PVP) and cetyltrimethylammonium bromide (AgNP-CTAB)] and silver nitrate (AgNO3) on tobacco (Nicotiana tabacum) seedlings oxidative stress parameters. Proline, glutathione (GSH) and hydrogen peroxide (H2O2) levels were spectrophotometrically measured in seedlings treated with AgNPs or AgNO3 (25, 50 and 100 μmol/L). Cellular effects were analysed in roots and tobacco seedlings leaves treated with AgNPs or AgNO3 (100 μmol/L) by in situ detection of reactive oxygen species (ROS) using dihydroethidium for superoxide radical (O2˙-) and 2', 7'-dichlorodihydrofluorescein diacetate for H2O2. Cell viability was determined using propidium iodide. Only AgNP-CTAB treatments caused significant increase in proline and H2O2 content in extracts, while AgNP-PVP and AgNO3 induced higher GSH production. Increased cellular O2˙- accumulation was observed in roots of all treated seedlings and AgNP-CTAB treated leaves. On the contrary, H2O2 production increased in AgNP-PVP treated roots and leaves exposed to all silver forms (PVP- and CTAB-coated AgNPs, and ionic silver). Cell death was severe in both roots and leaves in all treatments. Obtained results show that both AgNP-PVP and AgNP-CTAB induce oxidative stress causing cellular damage through mechanisms that cannot be completely assigned to Ag+ release.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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